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Is there a risk that my exosomes will be damaged during ultracentrifugation?
Ultracentrifugation is the gold standard for isolating and purifying exosomes from biological fluids and cell culture samples. Some degree of sample loss can occur due to apoptosis and incomplete sedimentation, however ultracentrifugation enables robust and reliable isolation of exosomes, while minimizing co-purification of protein aggregates and other membranous particles. Care should be taken to avoid situations that can cause exosome samples to lyse, including vigorous agitation, detergents and freezing.
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