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When light passes through a substance, it tends to refract. As concentration changes, the fringe pattern shifts. This is the basis for a Rayleigh interferometer.
Light from a diode laser passes through a collimating lens, creating two parallel light waves. These waves pass through the reference buffer and sample in the analytical cell. A series of lenses focus the resulting image onto a charge-coupled device (CCD) camera, which digitally captures the resulting fringe pattern in a single snapshot.
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