Error Prevention at Start Up, Cleaning and Worklist Generation

The AQUIOS CL system employs several features that work to prevent errors during startup, cleaning, and worklist generation. These prevention methods can be broken down into the following:

Table 2. Error Prevention Startup/Cleaning/Worklist Generation at a Glance

Autocleaning

Autocleaning is a function integrated directly into the AQUIOS system. The AQUIOS CL flow cytometer continually monitors and detects potential clogs and/or air bubbles in the flow cell. Upon detection of a potential clog or bubble in the flow cell, the system aborts the sample run and activates an autocleaning cycle. Once this cycle is complete, the system will rerun the aborted sample. Autocleaning reduces the potential for low precision and erroneous results. In addition, autocleaning reduces the need for sample reruns.

Test Requests to and from the LIS

Traditional flow cytometers do not automatically transfer test requests to and from the LIS without the added expense of middleware. If the LIS connection is enabled on the AQUIOS CL flow cytometer, the system automatically retrieves the test request from the LIS. Users with Reviewer access are authorized to review and transmit samples to the LIS. Reviewer users are able to generate and perform test requests, and review and release results.

Not only does the built-in LIS ensure automatic transfer of test requests reducing transcription errors, but no middleware is required. Different user access levels ensure that operators can only work on areas that align with their level of experience with the system.

Creating Worklists

On a traditional flow cytometer, there is no direct connection from the worklist to sample preparation system, or sample preparation system to the analyzer. Traditional flow cytometers automatically send the results from the analyzer to the LIS and the test request from the middleware to the analyzer when using middleware. Potential errors may result because everything is not linked together. Refer to Figure 1 for a visual representation of an LIS connection using the FC 500 flow cytometer and TQ-Prep sample prep system.

Figure 1. LIS Connection for FC 500 Flow Cytometer and TQ-Prep Sample Prep System

LIS Connection for FC 500 Flow Cytometer and TQ-Prep Sample Prep System

Conversely, the AQUIOS CL flow cytometer is one seamless system; no middleware is required. However, middleware can be used if desired. The system is able to send test requests to the analyzer and capture the information to automatically generate a worklist. Refer to Figure 2 for a visual representation of the LIS connection for the AQUIOS CL flow cytometer.

Figure 2. LIS Connection for the AQUIOS CL Flow Cytometer

LIS Connection for the AQUIOS CL Flow Cytometer

Fill the form to download Workflow Comparison Study with AQUIOS CL Flow Cytometer

London Health Sciences Centre (LHSC) Workflow Comparison Study with the AQUIOS CL Flow Cytometer

Контроль продвинутой стадии ВИЧ

Решения для контроля продвинутой стадии ВИЧ

Мониторинг количества CD4-лимфоцитов необходим для получения важной информации, от которой зависит лечение пациентов. Он позволяет врачам узнавать об изменениях в течении болезни, о ее стадии и принимать более взвешенные решения.

Быстрый и эффективный анализ субпопуляций лимфоцитов на AQUIOS CL

При клиническом ведении иммунодефицитных заболеваний точный подсчет абсолютного количества лейкоцитов в разных субпопуляциях и измерение доли отдельных субпопуляций в крови являются критически важными.

Сравнительный анализ систем AQUIOS CL PLG и FC500 MCL FlowCARE PLG

По итогам этого исследования можно сделать вывод о строгой корреляции результатов, полученных на двух системах.

Система проточной цитометрии AQUIOS CL Tetra и образцы цельной крови после длительного хранения

В исследовании показано, что проточный цитометр AQUIOS CL с реагентами Tetra обеспечивают точные результаты при анализе популяций T-, B- и NK-лимфоцитов в образцах крови здоровых и больных доноров, собранных в пробирки с ЭДТА K3 и хранившихся при комнатной температуре до 24 часов.

Error prevention during Startup and Worklist creation

The AQUIOS CL system employs several features that work to prevent errors during startup, cleaning, and worklist generation, including Autocleaning, Test Requests to and from the LIS and Creating Worklists.

Error Prevention During Sample Prep

The AQUIOS CL system employs several features that work to prevent errors during sample prep.

Error Prevention During Quality Control

The AQUIOS CL system employs several features that work to prevent errors during quality control, including Automatically Pause when QC Fails, QC Checks, Daily QC (Controls), Real Time QC (Patient Sample).

References:

  1. 1997 Revised Guidelines for the Performance of CD4+ T-Cell Determination in Persons with Human Immunodeficiency Virus (HIV) Infection. MMWR, January 10, 1997, p. 11.
  2. Levey S., Jennings, E.R., (1950) The use of control charts in the clinical laboratory. Am. J. Clin. Pathol. 20:1059.
  3. Hulspas R, Dombkowski D, Preffer F, Douglas D, Kildew-Shah B, Gilbert J. Flow Cytometry and the Stability of Phycoerythrin-Tandem Dye Conjugates. International Society for Advancement of Cytometry. September 23, 2009.
  4. Hulspas R, O’Gorman MRG, Wood BL, Gratama, JW, Sutherland DR. Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry Part B 2009;76B:355-364.
  5. Centers for Disease Control and Prevention. 2003 Guidelines for performing single-platform absolute CD4+ T-cell determinations with CD45 gating for persons infected with Human Immunodeficiency Virus HIV). MMWR 2003; 57 (No. RR. 2): 1-13.