Протоколы

В зависимости от области вашего исследования вам, возможно, не придется разрабатывать новый экспериментальный протокол с нуля.

Мы разработали библиотеку проверенных протоколов для использования с инструментами Beckman Coulter.

Выделите несколько минут на просмотр этого списка протоколов. Так вы можете сэкономить свое время на создание собственного протокола.

Название	Описание
DNA extraction from up to 2 mL blood using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Here we present a highquality, research-ready DNA extraction method from large volume whole blood by using GenFind V3.
DNA extraction from Zeesan@ Saliva DNA sample collection kit using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Saliva contains buccal epithelial cells and white blood cells which provide rich genetic data. Due to the easy access and non-invasive character, saliva is an alternative to blood collection. Here we present a high-quality, DNA extraction method from saliva using GenFind V3.
Isolation of RNA from Bacteria using RNAdvance Tissue Kit {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol optimizes RNA isolation from a wide range of bacteria using RNAdvance Tissues with lysozyme
Simultaneous DNA and RNA Extraction from Tissue without Splitting Lysate {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	In this protocol we developed a protocol using Beckman Coulter reagents to isolate both DNA and RNA from the same fresh/frozen sample. The method utilizes size selection to separate DNA and RNA
Apostle MiniMax™ High Efficiency cfDNA Isolation Kit - 1mL 50 Preps {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Protocol (version 35): Isolation of DNA from cell-free plasma, serum, and urine samples.
DNA Extraction from Filamentous Fungi using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol provides two other methods for lysis that are more automation friendly and we show that they produce results that are very similar to the industry standard of bead beating.
Apostle MiniMax™ High Efficiency cfDNA Isolation Kit - 1mL 10 Preps {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Protocol (version 35): Isolation of DNA from cell-free plasma, serum, and urine samples.
Total Nucleic Acid Isolation from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues using FormaPure XL Total on a KingFisher™ Duo Prime {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol demonstrates the FormaPure XL Total performance on the KingFisher Duo Prime purification system. Automating chemistry can reduce the risk of human error, hands-on time (HOT) and total turn-around time (TAT).
DNA Isolation from Bacteria using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol provides a method for extracting DNA from bacteria with data showing its effectiveness with Escherichia coli, Staphylococcus aureus, Salmonella, Corynebacterium and Mycobacterium, a difficult to work with bacteria
Mini prep of plasmids using CosMCPrep {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol provides a method that takes under 30 minutes to prep a plasmid.
DNA extraction from Oragene® Dx OGD-600 Saliva collection tubes using DNAdvance {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Saliva contains buccal epithelial cells and white blood cells that provide rich genetic data. Due to the easy access and non-invasive character, saliva is an alternative to blood collection. Here we present a high-quality DNA extraction method from saliva using DNAdvance.
Whole Blood DNA Extraction from PAXgene Blood DNA Tube using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Here we show that the GenFind V3 protocol can process whole blood directly from PAXgene™ Blood DNA Tubes with no centrifugation step necessary
DNA extraction FTA cards or filter paper blood spots using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This method provides instruction for additional lysis buffer volumes and optimized lysis conditions for extraction of DNA
DNA extraction from Mouthwash samples using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Mouthwash samples are taken in large volumes (usually 10 - 30 mL), and cells need to be spun down from the total volume of mouthwash before extraction can begin. This protocol incorporates those steps and optimizes the lysis for mouth cell samples
Extraction of DNA from Mucoid Bacteria using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol reduces viscosity due to the presence of exopolysaccharides and decreases the amount of polysaccharide in the final eluate.
Formalin-fixed and non-paraffin-embedded tissue DNA extraction {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Here we demonstrated a DNA extraction protocol from formalin-fixed and nonparaffin-embedded brain tissue using FormaPure DNA XL kit. In this protocol, protein-DNA cross-links are reversed using heat
DNA extraction from fresh/frozen tissue using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This provides a solution that allows a user to use one kit to extract DNA from multiple sample types with small modifications in the workflow.
RNA Isolation from Mucoid Bacteria with RNAdvance Tissue Kit {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol significantly reduces viscosity due to the presence of exopolysaccharides and decreases the amount of polysaccharide in the final eluate.
GenFind V2 Supplemental Protocol: Isolation of gDNA from Large Number of Cells {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol provides a method for extracting intact, high quality gDNA, while being scalable and offering the ability to be automated on Beckman Coulter liquid handler
Cell-Free DNA (cfDNA) Isolation from Plasma using Apostle MiniMax™ High Efficiency cfDNA Isolation Kit on a King Fisher™ Duo Prime {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol demonstrates the Apostle MiniMax High Efficiency cfDNA Isolation Kit performance on the KingFisher Duo Prime purification system.
DNA extraction from cell culture using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol provides a method for extracting DNA from cultured cells (≤ 2 million cells). Data shown shows the efficiency of this procedure with 1.4 million Jurkart cells, an immortalized line of human T lymphocyte cells
Apostle MiniMax™ High Efficiency cfDNA Isolation Kit - 5mL 50 Preps {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Protocol (version 35): Isolation of DNA from cell-free plasma, serum, and urine samples.
Apostle MiniMax™ - A new scalable and automatable method for the extraction of cfDNA {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	In this poster we present a novel cfDNA extraction kit and show its compatibility with extractions from 200 µl – 5 mL.
Isolation and Separation of DNA and RNA from a Single Tissue or Cell Culture Sample {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	We are currently developing a product using proprietary bead based technology that selectively binds DNA to the beads to effectively separate DNA and RNA independently. This allows for the extraction of all nucleic acids from one experimental setup
CosMCPREP DataSheet - High and Low Copy Plasmid Purification {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	CosMCPrep uses a single protocol to purify a variety of high and low copy number templates while maintaining the flexibility to support bothmanual and automated processing.
Apostle MiniMax Datasheet {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Apostle MiniMax™ High Efficiency cfDNA Isolation Kit, Apostle MiniMax is a cell–free DNA (cfDNA) isolation reagent kit, built on magnetic bead–based technology. Apostle MiniMax has been demonstrated to purify cfDNA from human plasma in both manual and automated workflows.
AMPure XP Datasheet {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Maximizing recovery, consistency, and speed to facilitate the entire NGS workflow, AMPure XP is optimized to meet the stringent needs of today’s genomic applications and to minimize the risk of losing important genetic information
SPRI Select DataSheet {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	The SPRISelect Reagent Kit is designed to give scientists more flexibility and control over the size selection process. With proven, industry leading Solid Phase Reversible Immobilization (SPRI)technology, this reagent provides robust, reproducible and customizable size selection.
DNAdvance DataSheet {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	The DNAdvance kit offers greater recovery of high quality gDNA than other commercially available DNA extraction kits. The extraction can be run manually in a 2 mL tube format or 96-well format, or automated in 96-well format on variety of Beckman Coulter Biomek liquid handling workstations.
RNAdvance Tissue DataSheet {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	The RNAdvance Tissue kit is a ribonucleic acid (RNA) isolation reagent kit built on SPRI paramagnetic bead-based technology. It enables extraction of total RNA from a wide variety of tissues without the hazards and waste removal issues of organic solvents or the time and labor consuming steps of vacuum filtration and centrifugation procedures.
GenFind V3 DataSheet {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	The method can be run manually in a 2 mL tube format or 96-well format, or automated in 96-well format on variety of Beckman Coulter Biomek liquid handling workstations
Total RNA isolation from >50,000 cells {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	A supplemental method utilizing SPRI technology to effectively and efficiently isolate total RNA from large cell numbers.
Isolation of DNA from Buccal Swabs using DNAdvance Kit	This protocol increases the volume of lysis buffer so that these two swabs from a single individual can be run in a single well and have the swab heads covered by lysis buffer.
Simultaneous DNA & RNA Extraction from Cultured Cells without Splitting Lysate {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	We developed a protocol using Beckman Coulter reagents to isolate both DNA and RNA from the same cultured cell sample. The method utilizes a proprietary buffer to selectively bind RNA and DNA