BioLector XT Microbioreactor
Microfluidic Bioprocess Control
Cultivation conditions
| 10 – 50 °C (min. temp.: 8 °C below ambient temp.) |
100 – 1500 rpm (3 mm diameter)
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Active humidification
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Ambient air
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1 – 100 % O2 (optional)
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0 – 12 % CO2 (optional)
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Anaerobic cultivation (optional)
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| 0 – 100 % dissolved oxygen*1 |
pH 4 – 7.5 (depending on plate)
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2.7 min / filter / 48 wells @ 1000 rpm
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Microfluidic features*2
pH control range: 4.0 – 7.5 (depending on plate)
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| Fully editable PI control |
Slow, medium and fast PI default settings
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Two sided pH control (alkali and acid)
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One sided pH control and one feed line (alkali or acid + one feed)
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Two feed lines
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Profile equation: dV/dt =A+B×t+C×eD×t
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Constant: A [μL/h]
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Linear: A [μL/h] and B [μL/h²]
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Exponential: A [μL/h], B [μL/h²], C [μL/h] and D [h-1]
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Pulse feed
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Full feeding profile flexibility permits broad experimental design
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| Up to 665 pump strokes per hour |
System dimensions
Gassing lid dimensions
Available optional modules
E-XTMF
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TradeGothicLT-Extended
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Active control of pH according to online signals and continuous feeding of up to two solutions |
only with Microfluidic plates
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E-O2XT-100
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O2 up-regulation module
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Control of gas atmosphere in head space: 21 – 100 % O2
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E-O2XT-25
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O2 down-regulation module
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Control of gas atmosphere in head space: 1 – 21 % O2
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use only with N2
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E-CO2XT-12
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CO2 up-regulation module
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Control of gas atmosphere in head space: 0 – 12 % CO2
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E-AN-300
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Anaerobic cultivation module
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Gassing with 100 % N2 allows cultivation of organisms in anaerobic conditions
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use only with N2
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All optional modules compatible in one BioLector microbioreactor device.
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Microtiter plates
48 cultivation wells
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Filling volume: 800 – 1900 μL (rpm dependent)
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High OTR and high kLa
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48 cultivation wells
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Filling volume: 1000 – 2400 μL (rpm dependent)
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Lower OTR and low shear force
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Available as both FlowerPlate and Round Well Plate
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32 cultivation wells controlled by 16 reservoir wells
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Maximum filling volumes in reservoir wells: 1800 µL (FlowerPlate) and 2000 µL (Round Well Plate)
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Same filling volumes for cultivation wells as in 48 well plate
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Lab space and material requirements
Flat surface with a minimal loading capacity of 100 kg for BioLector XT microbioreactor or 250 kg for BioLector XT microbioreactor with the valve control unit*2
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Device weight: 58 kg for BioLector XT microbioreactor (61 kg with microfluidic module) and 44 kg for valve control unit*2
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1x power supply for BioLector XT microbioreactor: 90-264 VAC, 47~63 Hz 1x power supply for laptop: 90-230 VAC, 50/60 Hz 1x power supply for valve control unit*2: 90-264 VAC, 47~63 Hz (US/Canada); 85-264 VAC, 47~63 Hz (EU, ROW)
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Microfluidics: requires 4 to 6 bar dry and oil-free compressed air, 6 mm ØOUT push-in connection
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Gassing modules (O2 up, O2 down, CO2 up, anaerobic module): require 1.5-2 bar dry and oil-free O2, CO2, or N2; 4 mm ØOUT push-in connection
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Humidity control: 400 mL deionized water
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*1 100 % corresponding to the DO level reached while gassing with 100 % O2 without O2 consumption
*2 only with optional microfluidic module OTR: Oxygen transfer rate [mmol/L/h]
kLa: Volumetric oxygen transfer coefficient [h-1]
m2p and the m2p logo are the trademarks or registered trademarks of m2p-labs GmbH in the US and other countries.
m2p-labs is a Beckman Coulter company.
Additional BioLector XT Content
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