HIAC PODS+ Online Mode & Filter Cart Mode
Joe Dabbs & Bill F. Bars | Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USAAbstract
The HIAC PODS+ has three different sampling modes, Bottle Mode, Online Mode and Filter Cart Mode. The Bottle Mode is for sampling fluid that is contained within a bottle and then the bottle is placed in the sample cup chamber. Online Mode is when you connect the HIAC PODS+ directly to a system and the instrument makes measurements periodically. Filter Cart Mode is identical to Online Mode but with added features, like controlling an external device or simply turning a light on when the fluid becomes dirty. Knowing the differences between Online Mode and Filter Cart Mode will allow operators to get the most out of their PODS+.
Difference between Online Mode and Filter Cart Mode
Both are online configurations of the PODS+ instrument where the instrument is connected directly to an active fluid system:
Online Mode
Makes measurements periodically from an active system to determine cleanliness in a dynamic environment.

Filter Cart Mode
Identical to Online Mode with the exception of the provision to specify a desired channel count level or cleanliness code at a point the operator would like to control an external device when that count or code is reached. This can be utilized for both “Clean to Dirty” or “Dirty to Clean”, scenarios.

*Waste line must have little or no pressure for fluid to properly flow through the PODS+
Scenario 1 – Filter Cart Mode Clean to Dirty
Connect the PODS+ to an active system and taking a particle count measurement hourly. Like most systems, contamination levels increase steadily while the system is in operation. The operator would like to know either visually or audibly without constantly monitoring the instrument when the contamination level reaches an ISO code of 20 on the 4µm channel. The PODS+ can be connected in the online manner to the system to perform the periodic sample testing. The Filter Cart Mode is selected and programmed to trigger an external device when the system reaches the desired code of 20 on the 4µm channel (Clean to Dirty option). Once the desired code is reached, the PODS+ is equipped with an I/O control port with relay contact control to drive an alarm light, audible alarm, or other electronic device to signal the operator the system is dirty and in need of service.
Scenario 2 – Filter Cart Mode Dirty to Clean
This is a follow onto the above scenario where now you have a dirty system that needs to be connected to a filter cart for cleaning. The PODS+ can be configured with the “Dirty to Clean” option and a cleanliness trigger level of an ISO code of 14 on the 4µm channel. Start the system and start the PODS+ sampling. The I/O port would be connected to control the filter cart pump so that when the cleanliness code of 14 on the 4µm channel is reached, and the system is sufficiently clean, the control signal can turn off the filter cart pump. This is a walk away scenario where the instrument, filter cart, and system are started simultaneously and when the process is complete the Filter cart pump is shut down to be disconnected and removed at the customer’s convenience.
Hardware
For both the Online and Filter Cart mode the following accessories are required. Online Adapter Kit Part number: B95366 Included in the Kit: On-Line Adapter, High Pressure Hose Mini-mess/SAE J514 40”, High Pressure Hose Mini-mess/Mini-mess 78”, Drain Tubing 6’, antistatic PTFE with metal quick connect, and a FFKM O-ring.
Helpful Links
-
Рекомендации по применению
- 17-Marker, 18-Color Human Blood Phenotyping Made Easy with Flow Cytometry
- 21 CFR Part 11 Data Integrity for On-line WFI Instruments
- 8011+ Reporting Standards Feature and Synopsis
- Achieving Compliant Batch Release – Sterile Parenteral Quality Control
- Air Particle Monitoring ISO 21501-4 Impact
- Революция в аналитическом ультрацентрифугировании: новая центрифуга Optima AUC
- Analyzing Mussel/Mollusk Propagation using the Multisizer 4e Coulter Counter
- Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry
- Автоматизированный анализ посева и роста клеток
- Automated Cell Transfection and Reporter Gene Assay
- Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi‑CELL XR
- Ускорение ваших геномных исследований с автоматизацией пробоподготовки на Biomek
- Ускорение ваших геномных исследований с автоматизацией пробоподготовки на Biomek
- Ускорение исследований с автоматизированными станциями Biomek для геномики
- Automated salt-assisted liquid-liquid extraction
- Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS
- Automated Transfection Methods
- Automated XTT Assay for Cell Viability Analysis
- Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex
- Automating Biopharma Quality Control to Reduce Costs and Improve Data Integrity
- Automating Bradford Assays
- Automating Cell Line Development
- Automating Cell Line Development
- Automating Cell-Based Processes
- Automating the Cell Line Development Workflow
- The new Avanti J-15 Centrifuge Improves Sample Protection and Maximizes Sample Recovery
- The New Avanti J-15 Centrifuge Time Saving Deceleration Profile Improves Workflow Efficiency
- Комплексное решение для очистки белков с центрифугой Avanti JXN
- Avoid the Pitfalls When Automating Cell Viability Counting for Biopharmaceutical Quality Control
- Beer, Evaluation of Final Product and Filtration Efficiency
- Biomek Automated Genomic Sample Prep Accelerates Research
- Biomek Automated NGS Solutions Accelerate Genomic Research
- Preparation and purification of carbon nanotubes using an ultracentrifuge and automatic dispensing apparatus, and analysis using an analytical centrifuge system
- Cell Counting Performance of Vi–Cell BLU Cell Viability Analyzer
- Cell Line Development – Data Handling
- Cell Line Development – Hit Picking
- Cell Line Development – Limiting Dilution
- Cell Line Development – Selection and Enrichment
- Changes to GMP Force Cleanroom Re-Classifications
- Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation
- Clean Cabinet Air Particle Evaluation
- Cleanroom Routine Environmental Monitoring – FDA Guidance on 21 CFR Part 11 Data Integrity
- Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System
- Conducting the ISO 14644-3 Cleanroom Recovery Test with the MET ONE 3400
- Considerations of Cell Counting Analysis when using Different Types of Cells
- Consistent Cell Maintenance and Plating through Automation
- Control Standards and Method Recommendations for the LS 13 320 XR
- Counting Efficiency: MET ONE Air Particle Counters and Compliance to ISO-21501
- Critical Particle Size Distribution for Cement using Laser Diffraction
- CytoFLEX
- Detecting Moisture in Hydraulic Fluid, Oil and Fuels
- Determination of Size and Concentration of Particles in Oils
- Efficient kit-free nucleic acid isolation uses a combination of precipitation and centrifugation separation methods
- dsDNA Quantification with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings
- Compensation Setup For High Content DURAClone Reagents
- Echo System-Enhanced SMART-Seq v2 for RNA Sequencing
- Efficient Factorial Optimization of Transfection Conditions
- Решения для производства и разработки вакцин
- Enumeration And Size Distribution Of Yeast Cells In The Brewing Industry
- European Pharmacopoeia EP 2.2.44 and Total Organic Carbon
- Evaluation of Instrument to Instrument Performance of the Vi-CELL BLU Cell Viability Analyzer
- Exosome-Depleted FBS Using Beckman Coulter Centrifugation: The cost-effective, Consistent choice
- Flexible ELISA automation with the Biomek i5 Workstation
- Full Automation of the SISCAPA® Workflow using a Biomek NXP Laboratory Automation Workstation
- Fully-Automated Cellular Analysis by Flow Cytometry
- Соответствие требованиям GMP
- g-Max: Added Capabilities to Beckman Coulter's versatile Ultracentrifuge Line
- Grading of nanocellulose using a centrifuge
- A method of grading nanoparticles using ultracentrifugation in order to determine the accurate particle diameter
- Grading of pigment ink and measurement of particle diameter using ultracentrifugation / dynamic light scattering
- HIAC Industrial – Our overview solution for fluid power testing for all applications
- HIAC PODS+ Online Mode & Filter Cart Mode
- HIAC PODS+ versus Parker ACM-20 Performance comparison
- A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System: an application for robust and scalable cancer research and biomarker discovery
- A Highly Consistent BCA Assay on Biomek i-Series
- A Highly Consistent Bradford Assay on Biomek i-Series
- A Highly Consistent Lowry Method on Biomek i-Series
- Highly Reproducible Automated Proteomics Sample Preparation on Biomek i-Series
- Cell Line Development – Hit Picking
- How to Use Violet Laser Side Scatter Detect Nanoparticle
- How Violet Side Scatter Enables Nanoparticle Detection
- HRLD Recommended Volume Setting
- Hydraulic Oil Measurement
- ICH Q2 – the Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems
- ICH Q2 – The Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems
- Importance of TOC measurement in WFI in light of European Pharmacopoeia change
- Temperature dependence of hydrodynamic radius of an intrinsically disordered protein measured in the Optima AUC analytical ultracentrifuge.
- Isolation of cell-free DNA (cfDNA) from plasma using Apostle MiniMax™ High Efficiency cfDNA Isolation kit— comparison of fully automated, semi-automated and manual workflow processing
- Issues with Testing Jet Fuels for Contamination
- Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity
- Линейность результатов изучения олигомеризации БСА методом AUC с использованием оптики поглощения и интерференции
- Long Life Lasers
- LS 13 320 XR: Sample Preparation - How to measure success
- Particle Size Analysis Simple, Effective and Precise
- Matching Cell Counts between Vi–CELL XR and Vi–CELL BLU
- MET ONE Sensor Verification
- Metal colloid purification and concentration using ultracentrifugation
- Separation and purification of metal nanorods using density gradient centrifugation
- Method for Determining Cell Type Parameter Adjustment to Match Legacy Vi CELL XR
- Minimal Sample to Sample Carry Over with the HIAC 8011+
- Minimizing process variability in the manufacturing of bottled drinking water
- Modern Trends in Non‐Viable Particle Monitoring during Aseptic Processing
- Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin
- Particle diameter measurement of a nanoparticle composite - Using density gradient ultracentrifugation and dynamic light scattering
- What to do now that ACFTD is discontinued
- Optimizing the HIAC 8011+ Particle Counter for Analyzing Viscous Fluids
- Optimizing the Multisizer 4e Coutler Counter for use with Small Apertures
- Optimizing Workflow Efficiency of Cleanroom Routine Environmental Monitoring
- Particle Counting in Mining Applications
- Particle testing in cleanroom high-pressure gas lines to ISO 14644 made easy with the MET ONE 3400 gas calibrations
- Pharma Manufacturing Environmental Monitoring
- Pharma Manufacturing Paperless Monitoring
- Принцип проточного центрифугирования
- Комплексное решение для очистки белков с центрифугой Avanti JXN
- Background Subtraction
- Calibrating the QbD1200 TOC Analyzer
- Detection Limit
- Inorganic Carbon Removal
- JP SDBS Validation
- Method Overview
- Overload Recovery
- QbD1200 Preparing Reagent Solution
- USP System Suitability
- Quality Control Electronic Records for 21 CFR part 11 Compliance
- Using the Coulter Principle to Quantify Particles in an Electrolytic Solution for Copper Acid Plating
- RCC A standardized automated approach for exosome isolation and characterization
- RCC Avanti J 15 Series Maximizing Sample Protection and Sample Recovery
- RCC Avanti J 15 Series Time Savings and Workflow Efficiency
- Root Cause Investigations for Pharmaceutical Water Systems
- Scalable Plasmid Purification using CosMCPrep
- Specification Comparison of Vi–CELL XR and Vi–CELL BLU
- Specifying Non-Viable Particle Monitoring for Aseptic Processing
- A Standardized, Automated Approach For Exosome Isolation And Characterization Using Beckman Coulter Instrumentation
- Switching from Oil Testing to Water and back using the HIAC 8011+ and HIAC PODS+
- Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube-based DURAClone dry reagent
- Using k-Factor to Compare Rotor Efficiency
- USP 787 Small Volume Testing
- Validation of On-line Total Organic Carbon Analysers for Release Testing Using ICH Q2
- Vaporized Hydrogen Peroxide Decontamination of Vi–CELL BLU Instrument
- Vi CELL BLU FAST Mode Option
- Vi-CELL BLU Regulatory Compliance - 21 CFR Part 11
- A workflow for medium-throughput isolation of cfDNA from plasma samples using Apostle MiniMax™ on the KingFisher™ Technology