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Early Discovery

Название	Описание
CytoFLEX {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Echo System-Enhanced SMART-Seq v4 for RNA Sequencing	Technical Note: We document information and results pertaining to the miniaturization of this process utilizing the Echo 525 Liquid Handler, effectively reducing the reagent cost and processing time for the workflow, addressing current throughput needs of RNA sequencing.
Echo System-Enhanced SMART-Seq v2 for RNA Sequencing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: We demonstrate miniaturization of this process on human brain total RNA utilizing the Echo 525 Liquid Handler.
MET ONE Particle Sensors Resistance to Sensors Resistance to Peroxide (VHP) {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Ted Lawrence \| Global Marketing Manager for Beckman Coulter Life Sciences \| Published 2013
Automated ELISAs—Save Time and Increase Throughput {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We demonstrate how the integration of the MultiWash™+ Microplate Washer and EMax® Plus Microplate Reader to a Biomek NXP Workstation facilitates the automated processing and analysis of ELISAs.
Cell Line Development – Data Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell line development is one of many workflows that has significant data challenges
HIAC PODS+ Online Mode & Filter Cart Mode {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Joe Dabbs & Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2017
Get Control in GMP Environments {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Floor-standing ultracentrifuges and high-performance centrifuges are vital to many bioproduction processes
Flow Cytometric Approach to Probiotic Cell Counting and Analysis Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes a staining procedure and instrument set up for absolute counting of probiotic bacterial culture. A workflow comparison with the traditional plate-based assay as well as correlated cell counts are provided.
Meet the Demand of Illumina’s High-Capacity NGS Platforms with Biomek Automated Sample Preparation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Improve Sample Quality with Standardized Sample Prep \| Quick Implementation with Demonstrated Methods \| Increase Throughput to Maximize Sequencing Runs
Automated Optimization of Cell Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we demonstrate how the Biomek FXP Workstation can be used to identify the optimal conditions for siRNA transfection in a single experiment.
Lentiviral Vector Preparation Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Viral vectors are one of the most commonly used tools for genetic manipulation in mammalian cells
Illumina Nextera Flex for Enrichment on the Biomek i7 Hybrid Genomics Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this flyer, we describe and demonstrate the automated performance of the Illumina Nextera Flex for Enrichment assay on the Biomek i7 Hybrid Genomics Workstation
Achieving Compliant Batch Release - Sterile Parenteral Quality Control {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Tony Harrison, Compliance and Applications Specialist \| Beckman Coulter, Inc., United Kingdom \| Published 2016
Isolation of cell-free DNA (cfDNA) from plasma using Apostle MiniMax™ High Efficiency cfDNA Isolation kit comparison of fully automated, semi-automated and manual workflow processing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note compares workflows and yield for the extraction of cfDNA using Apostle MiniMax™ High Efficiency cfDNA Isolation Kit, following the manual protocol, automating the extraction using the Biomek i7 Hybrid Workstation, and semi-automating the extraction using the KingFisher™ Duo Prime Sample Purification System.
Instrument-to-Instrument Variability in the Vi-CELL Automated Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Clarence Lew, Senior Applications Scientist, John A. Gomez, Senior Staff Technical Operations Engineer, Matthew N. Rhyner, Ph.D., Global Marketing Manager-Analytical Products \| Beckman Coulter Life Sciences, Brea, CA USA \| Published 2012
Using k-Factor to Compare Rotor Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The k-factor is a common parameter that describes the efficiency of a centrifuge-rotor system, although temperature is still overlooked
Automated Optimization of Cell Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Demonstrated application on how the Biomek FXP Workstation can be used to identify the optimal conditions for siRNA transfection in a single experiment.
Automated Illumina TruSeq Nano DNA Sample Preparation on the Biomek 4000 Laboratory Automated Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: David Horvath, M.S. - Beckman Coulter, Inc. \| Published 2016
Quantification of Cellular Toxicity with Particle Characterization and Centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Beckman Coulter tools enable quantification of cellular toxicity caused by nanoparticles, reducing time to remove aggregated particles and quantifying cell toxicity in the presence of single-walled carbon nanotubes.
Enzymatic Determination Of Glycerol Contents In Biological Samples Using The Biomek 4000 Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr. Jörg Mampel, B.R.A.I.N Aktiengesellschaft, Darmstaedter Str. 34-36, D-64673 Zwingenberg \| Published 2014
LS13320XR Laser Diffraction Particle Size Distribution Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sample Preparation – How to measure success!
Workflow Questions Answered: Challenges with FFPE Tissue Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Jung Doh, Ph.D., Senior Applications Scientist, Beckman Coulter, Inc. \| Published 2017
Particle Characterization & Centrifugation - Nanotoxicity Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nanotechnology biomedicine is an emerging field still in its nascent phase
Impact of Clean-Up Kits on DNA Sequencing Quality and Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The applications of DNA sequencing in biological research are growing. A significant driver of this growth is Next-Generation Sequencing (NGS), a modern DNA sequencing technology instrumental in achieving complete DNA sequences or genomes of humans, many animals, plants, and microbial species.
Rapid and easy intracellular + surface staining with PerFix-nc_MouseMarkers {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Rapid and easy intracellular + surface staining with PerFix-nc_MouseMarkers
Automated Solutions for Cellular Analysis Through Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski - Staff Applications Scientist, Li Liu - Senior Development Scientist, Amy Yoder - Staff Development Scientist, Beckman Coulter Life Sciences, Indianapolis, IN \| Published 2014
Scalable Plasmid Purification using CosMCPrep {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the flexibility of CosMCPrep, a scalable plasmid purification kit. CosMCPrep is shown here to work with volumes up to 5.5 mL purifying up to 300 µg of plasmid.
Automated Sample Preparation Improves Throughput for Flow Cytometric Analysis of Mouse Stem Cell Populations {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: William L. Godfrey, Li Liu, Amy Yoder, and Laura Pajak \| Beckman Coulter, Inc., Miami, FL, USA and Indianapolis, IN, USA
Biomek i-Series Automated Beckman Coulter Agencourt RNAdvance Cell {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Cell V2 Total RNA extraction kit uses Agencourt’s patented SPRI® paramagnetic bead-based technology to isolate total RNA from 200 to 50,000 cultured eukaryotic cells, including cell lines and primary cells.
Plant Genome Analysis by Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Provides a detailed protocol for preparing nuclei from plant tissues. DNA in the nuclei is then stained with propidium iodide and the flow cytometer is used to assess DNA content. Values obtained are then correlated with the Kew Database to assess ploidy. This assay can be used to investigate the role of endoreplication in plant development across species.
Miniaturization and Rapid Processing of TXTL Reactions Using Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: Using the Echo 525 Liquid Handler in combination with the myTXTL Master Mix provides a comprehensive solution for gene expression projects that require rapid high-throughput processing, flexibility, and reproducibility in a low cost assay
Miniaturized 16S rRNA Amplicon Sequencing with the Echo 525 Liquid Handler for Metagenomic and Microbiome Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: We perform a standard Illumina 16S rRNA amplicon sequencing library preparation at miniaturized scale using the Echo 525 Liquid Handler.
Measuring Proliferation in Leukemia Cell Lines Using CFSE Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye which is cell permeable. It binds covalently to intracellular proteins. Fluorescence decreases over time as the dye is divided between daughter cells at each division. In this application note a detailed protocol for using this assay to assess cytotoxic effects of a pharmacologic agent is described.
Using Pressure to Reduce Bubble Contamination from Particle Count Results {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Dave Dunham \| Beckman Coulter, Inc, Grants Pass, OR \| Published 2016
Sieves to Laser Diffraction...Hours to Seconds. See What You've Been Missing! {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Published 2013
MoFlo™ Viability and Function with High-Speed Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sustained cell sorting at speeds in excess of 50,000 events per second is routine when using the MoFlo high-performance cell sorter
Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, PhD, Application Scientist \| Beckman Coulter, Inc., Indianapolis, IN USA \| Published 2014
Density Gradient Separation of Gold Nanorods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Gold Nanorods (AuNR) hold great promise for biomedical imaging
cell-viability-vi-cell-blu-cell-type-analysis-types-201904-german {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Use of Beckman Coulter Reagents for Automated, Whole Blood, White Blood Cell Viability Measurements {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Stephen E. Szabo, Ph.D. \| Sarah Monroe, B.S.\| Beckman Coulter, Inc \| Published 2004
Optimizing the HIAC 8011+ Particle Counter for Analyzing Viscous Fluids {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Sr. Application Scientist for Beckman Coulter Life Sciences in Grants Pass, Oregon, USA \| Published 2013
High-throughput qPCR and RT-qPCR Workflows Enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB® Luna® Reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: We show that NEB Luna qPCR reagents offer strong performance in high-throughput qPCR applications and are readily compatible with automated reaction setup, here using the Beckman Echo 525 acoustic liquid handler.
A workflow for medium-throughput isolation of cfDNA from plasma samples using Apostle MiniMax™ on the KingFisher™ Technology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of Apostle MiniMax™ High Efficiency cfDNA Isolation Kit, in conjunction with the KingFisher Duo Prime automated protein purification system.
Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note: We used the Biomek FXP Workstation to automate the culture and drug sensitivity screening of cancer spheroids in Perfecta3D® Hanging Drop Plates
Efficient Factorial Optimization of Transfection Conditions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nucleic acid transfection is a procedure used in nearly any cellular laboratory and the abundance of commercially available reagents has made this a seemingly simple endeavor
dsDNA Quantification with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Echo 525 Liquid Handler was used to miniaturize and optimize these fluorescent-based quantification assays
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
Background Subtraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	When measuring a water sample to quantify TOC (Total Organic Carbon), most TOC analyzers add some combination of reagents and dilution water to the initial sample in order to complete the measurement
Optimizing Cell Separation with Beckman Coulter's Centrifugal Elutriation System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In many applications of cell biology, separation of cells from a heterogenous population to an enriched population of specific target cells is necessary to answer relevant scientific questions.
Overload Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In a typical laboratory environment, a TOC analyzer is used to measure water samples where the TOC value of those samples is unknown
HIAC 8011+ Using Pressure To Reduce Entrained Air from Fluid Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Dave Dunham \| Global Marketing Manager for Beckman Coulter Life Sciences \| Published 2013
Isolation of DNA from Buccal Swabs using DNAdvance Kit	This protocol increases the volume of lysis buffer so that these two swabs from a single individual can be run in a single well and have the swab heads covered by lysis buffer.
Biomek i-Series1 Automated FormaPure DNA Extraction kit for FFPE Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissue is essential for many cancer research applications
The New Avanti J-15 Centrifuge Improves Sample Protection and Maximizes Sample Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	By focusing on sample protection, the Avanti J-15 series has reduced sample disturbance during acceleration and deceleration, increasing sample yield, decreasing contamination and increasing centrifugation efficiency.
Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Three-dimensional (3D) cell cultures offer greater physiological relevance than monolayer cultures for cellular interaction studies and compound screens.
Efficient Kit-Free Nucleic Acid Isolation uses a Combination of Precipitation and Centrifugation Separation Methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Julia P Luciano-Chadee, Beckman Coulter, Inc., Indianapolis, IN 46268 \| Published 2017
Enhancing Vaccine Development and Production {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, PhD, Senior Application Scientist \| Beckman Coulter, Inc., Life Sciences, Indianapolis, IN USA \| Published 2015
Beckman Coulter’s New Line of Centrifuges Provides Support for 21 CFR Part 11 Compliant Laboratories {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As technology and software advance, security and protection of data is becoming increasingly important to ensure a properly functioning lab environment
Characterizing RNA Nanoparticles by Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Chad T. Schwartz, Daniel Jasinski, and Peixuan Guo - Beckman Coulter, Inc., Indianapolis, IN \| University of Kentucky, Department of Pharmaceutical Sciences
High-Performance Automation and Centrifugation Preparation of Carbon Nanotubes for Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Single-Walled Carbon Nanotubes (SWCNT) experienced increasing interest in the past 15 years for semiconductors, fuel cells, and biomedical applications
Miniaturized Sequencing Workflows for Microbiome and Metagenomic Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: We introduce experiments of various sequencing workflows, miniaturizing them at least 10-fold.
Automated Size Selection using SPRIselect Beads and the Biomek FX Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Alexandre Bélisle, Vicky Arsenault, Belisle Tir, Alfredo Staffa Sylvie LaBoissière and Alexandre Montpetit - McGill University and Génome Québec Innovation Centre - Montréal, Québec, Canada
Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate: Biomek Wide Bore vs. Standard Pipette Tips {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Robert D Lund, Beckman Coulter Life Sciences
Automating Biologics Signature Peptide Quantitation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Ian Moore, Technical Marketing Manager \| SCIEX, Concord, Canada, Michael Kowalski, Staff Applications Scientist \| Beckman Coulter Life Sciences, Indianapolis, IN
The new LS 13 320 XR Extended Range 10 nm - 3500 μm {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note exhibiting the extended range of the LS 13 320 XR
JP SDBS Validation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	JP 16 specifies that when measuring a 500ppb standard of sodium dodecylbenzenesuflonate (SDBS), a TOC analyzer should recover a value of at least 450ppb (90% recovery)
Automated Data Management for Reliable Cell-Based Screening {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist \| Beckman Coulter Life Sciences, Indianapolis, IN Timothy Sherrill, Staff Systems Engineer \| Beckman Coulter Life Sciences, Indianapolis, IN Tara Jones-Roe, Marketing Manager \| Beckman Coulter Life Sciences, Indianapolis, IN
Biomek Automated Genomic Sample Prep Accelerates Research Biomek i-Series Automated Beckman Coulter AMPure XP PCR Purification System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Beckman Coulter AMPure XP kit is a highly efficient PCR purification system that delivers
Accurate, Rapid Sizing of C60 Fullerene Particles {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Joshua T. Robinson \| Published 2016
Identifying Brain Tumor Initiating Cells Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Tumors represent a heterogenous population. Enumerating cells with stem cell like properties via co-expression of markers provides insight into the evolution of the tumor over time and throughout treatment. Detailed procedure for processing brain tissue for culture and flow cytometric analysis is provided.
Automated FFPE DNA Extraction using the FormaPure DNA Extraction Kit using the Biomek Series of Automated Liquid Handlers from Beckman Coulter, Inc. {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. \| Beckman Coulter, Inc. \| Published 2016
Phenotypic Characterization of Human Natural Killer Cell Populations in Peripheral Blood Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes a 10 color, 13 marker panel to investigate the NK cell population, both phenotypically and functionally in human PBMC samples. The panel includes the use of an exclusion markers on a dump channel used to gate out non-NK cells. Deep phenotyping markers for the NK cell compartment are included and a comprehensive gating strategy is defined.
Cell Line Development – Selection and Enrichment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The quest to find cells that stably express and secrete antibodies or other protein products from a transfected pool of cells requires the screening of thousands of wells/cells
High-Performance Exosome Purification and Characterization via Density Gradient Ultracentrifugation and Dynamic Light Scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Exosomes purification and analyses comprise a fast evolving research area; more than 70% of published research on exosomes has been done within the last six years
Workflow protocols {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Workflow protocols
Application note for advanced analysis of data obtained with DURAClone RE CLB {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note for advanced analysis of data obtained with DURAClone RE CLB
Assessment of T-cell receptor repertoire by flow cytometry using the Beta Mark TCR Vβ repertoire kit and Kaluza analysis software {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Discover a rapid and robust solution to investigate the TCR Vβ repertoire using flow cytometry
Illumina TruSeq Stranded mRNA Sample Preparation Kit Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq® Stranded mRNA Sample Preparation Kit protocol converts mRNA from total RNA into a template sample of known origin
Effective Miniaturization of Illumina Nextera XT Library Prep for Multiplexed Whole Genome Sequencing and Microbiome Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: Here we detail a method to miniaturize reaction volumes utilized in the Illumina Nextera XT DNA library kit, thereby reducing the overall cost per reaction while maintaining equivalent data to the standard protocol.
Consistent Cell Maintenance and Plating through Automation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell culture is a valuable yet time-consuming aspect of basic biological research and drug discovery
Automation of the Illumina® TruSeq® DNA PCR-Free Sample Preparation Kit on the Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S.
Method Overview {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	A water sample initially contains two types of carbon: Total Inorganic Carbon (TIC) (from CO2 gas dissolved in H2O and dissolved carbonates in the water) and Total Organic Carbon (TOC) (from organic species)
Automated Illumina TruSeq DNA PCR-Free Sample Preparation on the Biomek 4000 Laboratory Automated Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: David Horvath, M.S. \| Beckman Coulter, Inc.
Biomek i-Series Automated New England Biolabs NEBNext® Ultra IITM DNA Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Obtaining high library yields with low input DNA concentration remains a substantial challenge in NGS library preparation.
Methode zur Bestimmung der Zelltypparameter Anpassung zum Abgleich mit dem Vi–CELL XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Mit der Einführung des neuen Vi–CELL BLU ist es nun möglich, den Plattenmodus für komplexere Versuchsanordnungen zu nutzen.
A High Recovery and High Purity Data Analysis Strategy for Rare Abnormal Plasma Cell Events using the DuraClone RE PC Tube {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr Agnieszka Blum- Charité Virchow Hospital, Stem Cell Facility, Berlin, Germany \| Dr Michael Kapinsky - Life Science, Beckman Coulter, Marseille, France
Determination of Size and Concentration of Particles in Oils {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The concentration and size distribution of particles in an oil sample may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method
Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. Beckman Coulter, Inc. \| Published 2014
Identifying Discrete Mouse Splenocyte and Peripheral Blood Cell Subpopulations Using Surface Markers Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Flow cytometry is extremely useful for detecting cell surface antigen expression, and thereby facilitates the characterization, identification, and isolation of discrete cell populations within heterogeneous samples. We describe a method to rapidly isolate and delineate CD3ε-positive T cell and CD19-positive B cell populations from murine spleen and peripheral blood samples.
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
HIAC PODS+ vs. Parker ACM-20 Performance Comparison {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
Particle-counting-application-note-enumeration-size-distribution-yeast-cells-brewing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
マルチプレックス全ゲノムシーケンシングおよびマイクロバイオームアプリケーションのためのIllumina Nextera XTライブラリ調製の効果的な微量化 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	研究コストと時間削減への貢献する、リアクションボリュームの微量化メソッドについて解説します。
Optimizing Workflow Efficiency of Cleanroom Routine Environmental Monitoring {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper examines a thoughtfully designed and well-executed environmental monitoring program that tests the controls put in place to maintain the cleanroom to the required Good Manufacturing Practices standards (e.g., CGMP, EU-GMP, and PIC/S).
Smart DNA Fabrication Using Sound Waves: Applying Acoustic Dispensing Technologies to Synthetic Biology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this paper, we describe the first application of this powerful technology to the rapidly developing field of synthetic biology, for DNA synthesis and assembly at the nanoliter scale using an Echo 550 acoustic dispenser
The Particle Analyzers Advancing Life Science Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Scientists share how automated cell counting is revolutionizing research
MoFlo™ Chromosome Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Flow cytometric chromosome classification (flow karyotyping) has been routinely used in the analysis of mammalian karyotypes as well as to assess chromosomal abnormalities
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Tissue Kit uses Beckman Coulter’s patented Agencourt SPRI paramagnetic bead-based technology to isolate total RNA (microRNA and mRNA).
Automated Optimization of IgG Production in CHO Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist - Beckman Coulter Life Sciences, Indianapolis, IN \| David Apiyo, Marketing Applications Manager - Pall ForteBio, Fremont, CA \| Rashi Takkar, Product Manager - Pall ForteBio, Fremont, CA \| Tara Jones-Roe, Marketing Manager -Beckman Coulter Life Sciences, Indianapolis, IN
Application note for advanced analysis of data obtained with DURAClone RE PC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note for advanced analysis of data obtained with DURAClone RE PC
The Lipemic Serum Clarification System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Lipemic Serum Clarifi cation System clears blood samples of lipemia caused by chylomicrons—fat particles 80–500 nm in diameter that impair accuracy of spectrophotometric analyses
Improved Sequencing Performance with Publication-Quality Data {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt CosMCPrep system is a versatile plasmid purification procedure
MoFlo Astrios EQ Enhanced Forward Scatter: Simultaneous Sorting of Micro- and Macro- Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The MoFlo Astrios EQ series provides solutions to investigate biological materials between 200 nm and 30 μm
Using The Agencourt ® AMPure® And Agencourt CleanSEQ® Methods To Manage A Variety Of Input Sample Quality {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we present sample purification methods that assist in addressing the varying input sample quality encountered by facilities performing PCR amplification and sequencing for SNP detection.
Avanti JXN Series - Complete Solution for Protein Purification Workflow {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Purified proteins are required for many proteomics applications such as X-ray crystallography, NMR, mass spec, and in vitro biochemical assays
MoFlo™ Astrios* Enhanced Forward Scatter+: 0.2 μm to 30 μm Dynamic FSC Detection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr. Carley D. Ross, Mike Morrell, Robin Morris, Blake McCarty, Alan Dean Ph.D. and Nicole Lewis \| Beckman Coulter, Inc., Flow Cytometry Business Center, Fort Collins, CO, USA
Particle Characterization - Nanotube Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Carbon nanotubes are an emerging material that has had increasing industrial application in material reinforcement and great future potential in electronics, energy storage and conversion, and medicine
Characterizing T Helper Cells Populations by Intracellular Cytokine Expression Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	T helper cells are distinguished functionally by their cytokine profile, their major effector molecules. This application note provides a procedure for assessing the cytokine profile using flow cytometry and demonstrates T Helper cell subtypes Th1, Th2, Th17 and a non-classic Th1 type.
Biomek i-Series Automated SurePlex PCR and VeriSeq PGS Library Prep for Illumina® {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The SurePlex PCR and VeriSeq PGS (Preimplantation Genetic Screening) Library Prep for Illumina® is used to prepare up to 12 single-index OR up to 24 dual-index, single-read libraries from SurePlex amplification.
Chymotrypsin: Characterization of a Self-Associating System in the Analytical Ultracentrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Paul Voelker and Don McRorie \| Beckman Coulter
Bewertung der Leistung des Vi–CELL BLU Zellvitalitäts analysator bei der Nutzung mehrerer Geräte {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Beckman Coulter Life Sciences freut sich, Ihnen den neuen Vi–CELL BLU Zellvitalitätsanalysator vorzustellen
Cell-Based Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell Culture is an essential workflow that enables studies to be performed in drug discovery and scientific research
Dekontamination des Vi-CELL BLU Geräts mit verdampftem Wasserstoffperoxid {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In vielen Laboren ist eine Dekontamination des gesamten Geräts gewünscht und im Fall des Umgangs mit
g-Max: Added Capabilities to Beckman Coulter’s Versatile Ultracentrifuge Line {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Chad Schwartz, Ph.D., Application Scientist I \| Randy Lockner, M.S., Centrifugation Marketing \| Randy Pawlovich, Director of Product Management & Strategy \| Beckman Coulter, Inc., Indianapolis, IN USA
Coulter Principle Short Course {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	What is a particle? According to Webster’s Dictionary a particle is “a minute quantity or fragment” or “a relatively small or the smallest discrete portion or amount of something
Application Note: Advanced analysis of data obtained with DuraClone RE PC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note for advanced analysis of data obtained with DuraClone RE PC
Issues with Testing Jet Fuels for Contamination! {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Joe Dabbs \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
Getting Started with Kaluza: Data Scaling and Compensation Adjustment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Learn how to adjust the data scaling and fluorescence compensation of flow cytometry data in Kaluza by using the Add All Plots function, Spillover and Logicle Sliders.
Illumina TruSight® HLA Sequencing Panel Automated on the Biomek FXP HLA SP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe the automation of the Illumina TruSight® HLA Sequencing Panel on the Beckman Coulter Biomek FXP HLA SP.
Scalable, Automation-friendly and High Throughput PCR Purification {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt CleanSEQ system, dye terminator removal.
Assessing T Cell Subpopulations by TCR Expression in PBMCs Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We outline a protocol using DURAClone™ IM panels, which are unitized, dry format, room temperature stable reagent cocktails, to delineate and quantify T cell subpopulations from a heterogeneous sample of isolated normal PBMCs based on TCR composition.
Stability of the Human Immunodeficiency Virus-1 Reverse Transcriptase Heterodimer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Jacob Lebowitz - University of Alabama, Birmingham, AL
Particle Characterization & Centrifugation - Nanotoxicity Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nanotechnology biomedicine is an emerging field still in its nascent phase.
Using Cell Impermeant DNA Binding Dyes for Viability Assessment in Multicolor Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Compares viability staining using DRAQ7, DAPI, and 7-AAD in a multicolor immunophenotyping panel. Attributes of each dye are evaluated to aid in selection during panel design.
Phagocytosis of Bacteria in a Whole Blood Assay Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes an opsonization assay which can be used to assess the phagocytic capabilities of immune cell in whole blood. The benefits of using multicolor flow cytometry for the assay is that different phagocytic potential across various immune cell types can be determined in the same sample.
Automating Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell line development is the process by which the cellular machinery is co-opted to manufacture therapeutic biologics or other proteins of interest
Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Julia P Luciano-Chadee - Beckman Coulter, Inc., Indianapolis, IN \| Chad Schwartz Ph.D. - Beckman Coulter, Inc., Indianapolis, IN
Single Cell Analysis of Autophagy Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Provides a detailed procedure for using the Cyto-ID Autophagy kit in a flow cytometry assay. Contains background information on the physical and chemical hallmarks of autophagy and the benefits of a single cell assay versus bulk analysis of this biological process.
A Standardized, Automated Approach for Exosome Isolation and Characterization using Beckman Coulter Instrumentation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D., Zach Smith, M.S. Beckman Coulter Life Sciences, Indianapolis, IN 46268
High Recovery of DNA for Downstream Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt Genfind v2 DNA purification system isolates and purifies genomic DNA (gDNA) from whole blood and serum
Automation of NGS Sample Size Selection using SPRIselect {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	To streamline the NGS sample preparation process, Beckman Coulter has recently launched the SPRIselect Reagent Kit to allow NGS sample size selection to be automated on Biomek liquid handling platforms for high throughput and high efficiency sample preparations.
HIAC Industrial - Our overview solution for fluid power testing for all applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details measuring fluid contamination by cleanliness standards like ISO 4406, SAE AS4059, and NAS 1638 for oil and hydraulic fluid applications.
Compensation setup for high content DURAClone reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Compensation setup for high content DURAClone reagents
MoFlo™ Fluorescence Resonance Energy Transfer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Fluorescent proteins have become a mainstay in the study of protein localization, protein-protein interaction and gene expression
Intelligent Biosafety: MoFlo Astrios Fully Integrated with the Biosafety Cabinet and Aerosol Evacuation System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As with any sorter that requires jet-in-air sorting, aerosols are created both from the sort and waste streams
Biomek i-Series Automated Illumina® Nextera XT DNA Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina Nextera XT DNA Library Prep Kit is used to prepare up to 96 indexed paired-end libraries for sequencing on Illumina platforms.
Calcium Flux Assay by Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The unique peristaltic sample delivery system of the CytoFLEX analyzer enables optimized measurements of transient changes in intracellular calcium in cells following agonist activation.
Automated DNA Library Construction Using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe the automation of the New England Biolabs NEBNext Ultra DNA Library Preparation Kit for Illumina on the Beckman Coulter Biomek FXP Dual-Arm Multichannel 96 and Span-8 automated liquid handler (Biomek FXP ).
High-Throughput Illumina® TruSeq® Nano DNA Library Construction on the Biomek FXP Dual-Arm Multi-96 and Span-8 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The manual preparation of large numbers of sequencing libraries, which can be labor-intensive and time-consuming, is a bottleneck for many laboratories
Centrifuge-application-note-intrinsically-disordered-protein-AUC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Using Laser Diffraction in Folk and Ward Phi Notation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Soil studies span a range of applications including agriculture, architectural planning, and historical environmental studies
CytoFLEX Application Note18 ParameterFinal0319 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Highly Reproducible Automated Proteomics Sample Preparation on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Automation can relieve the burden of sample preparation while also reducing the variability between users and across experiments
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sample quality is of utmost importance to genomic researchers to better ensure good results when running laborious and expensive processes such as Next Generation Sequencing.
Illumina TruSight® HLA Sequencing Panel Automated on the Biomek FXP HLA SP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith, MS, Senior Applications Scientist - Beckman Coulter, Inc. \| Nate Baird, PhD, Scientist, Illumina \| Brad Baas, PhD, Senior Scientist, Illumina \| Published 2015
Automated Sample Preparation for the Monitoring of Ethanol Metabolites in Urine by LC-MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we describe an LC-MS/MS method for the analysis of EtG and EtS in urine employing a simple, automated sample preparation methodology using a liquid handling-based automation system.
A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of FormaPure XL Total, a total nucleic acid extraction kit for FFPE samples, in conjunction with the KingFisher™ Duo Prime Sample Purification System as a potential solution that mitigates some of the challenges with FFPE workflows.
Matching Cell Counts between Vi-CELL XR and Vi-CELL BLU {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note explains the flexibility to adjust the cell type settings to match results obtained from an equivalent sample when run on the Vi-CELL XR on the Vi-CELL BLU.
High Throughput TruSeq Stranded mRNA Library Construction on the Biomek FXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith and Scott D. Michaels - The Center for Genomics and Bioinformatics - Indiana University \| Mary Blair - Life Science Division Beckman Coulter - Indianapolis, IN \| Published 2013
High Throughput TruSeq Stranded mRNA Library Construction on the Biomek FXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith \| Scott D. Michaels \| Mary Blair
Short Column Sedimentation Equilibrium Analysis for Rapid Characterization of Macromolecules in Solution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Thomas M. Laue University of New Hampshire Department of Biochemistry Durham, NH 03824
Automated XTT Assay for Cell Viability Analysis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	XTT assays are a simple way of determining the number of viable cells; however, a reliable assay requires a standard curve of cell dilutions and optimized incubation times
Agencourt CleanSEQ System - Dye Terminator Removal {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt CleanSEQ is a SPRID (Solid Phase Reversible Immobilization) paramagnetic bead-based sequencing purification system with a simple three-step protocol
Biomek i-Series Automated Illumina TruSeq Exome Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq Exome Library Prep kit is a workflow to generate indexed paired-end libraries and s specially designed to work with Formalin Fixed Paraffin Embedded (FFPE) samples.
Cell Line Development – Limiting Dilution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Limiting dilution is a traditional approach to achieve monoclonality
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissue is essential
CytoFLEX Instrument Set-up for Measuring Extracellular Vesicles Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes the instrument set up and verification steps required for measuring extracellular vesicles by flow cytometry. Instructions for reducing background particles found in buffers and reagents to enhance small particle detection.
Determine Hydrodynamic Size of Surfactant Micelles with DelsaMax CORE {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sodium Dodecyl Sulfate (SDS) is a commonly used amphiphilic surfactant with wide-ranging applications, including chemical disinfection to protein denaturation in SDS-PAGE gels, cell lysing for DNA extraction, and nanoparticle solubilization
Biomek i-Series Automated KAPA HyperPrep and HyperPlus Workflows {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The KAPA HyperPrep and HyperPlus Kits allow users to prepare DNA libraries compatible with Illumina sequencing platforms from a variety of sample types (including high-quality genomic DNA, FFPE derived DNA, etc.) and a wide range of input amounts (1 ng to 1 µg).
High-Capacity Next Generation Sequencing Platforms {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Meet the Demand of Illumina’s High-Capacity NGS Platforms with Biomek Automated Sample Preparation
Biomek i-Series Automated Illumina TruSeq® Stranded mRNA Sample Preparation Kit Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq® Stranded mRNA Sample Preparation Kit protocol converts mRNA from total RNA into a template sample of known origin.
Automation of the Illumina® TruSeq® DNA PCR-Free Sample Preparation Kit on the Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. Beckman Coulter Life Sciences, Indianapolis, IN USA \| Published 2014
MoFlo™ Stem Cell Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Purified populations of functional stem cells are of great interest to the biomedical community, both in the understanding of stem cell biology and in clinical transplant settings
MoFlo™ Bacterial Proliferation and PHB Production {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Population dynamics were follow ed during growth on phenol in response to adaptation processes of Cupriavidus necato, bacterial individuals morphologically nearly uniform, yet structurally and functionally diverse
Cell Culture Automating Cell Maintenance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell culture is an essential workflow that enables studies to be performed in drug discovery and scientific
Biological Applications for Multisizer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Aspirin Analogues and Flow of Erythrocytes through Narrow Capillaries
Efficient Factorial Optimization of Transfection Conditions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Design of Experiment (DOE) approach can be quickly automated on the Biomek i7 Automated Workstation to identify optimal transfection conditions for a variety
Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes results obtained using a liquid handling-based automation system for the sample preparation of compounds to be analyzed by LC-MS/ MS and applied to the monitoring of multiple classes of pharmaceutical and illicit drugs.
FoxP3 staining in human whole blood using PerFix-nc {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	FoxP3 staining in human whole blood using PerFix-nc
Fully-Automated Cellular Analysis by Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Direct integration of CytoFLEX Flow Cytometer to Biomek i-Series Automated Workstations enables complete automation of sample processing and data acquisition
Biomek i-Series Automated AmpliSeq for Illumina® Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The AmpliSeq for Illumina Library Prep Kit is used to prepare dual indexed libraries for targeted sequencing on Illumina platforms.
High Recovery of DNA for Downstream Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt Genfind v2 system, genomic DNA isolation kit.
Automated Liquid Handling for Cell Staining and Imaging {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we describe a variety of automated solutions to reduce the time, effort, and complexity associated with sample preparation workflows for cell imaging.
Biomek i-Series Automated Illumina TruSeq® Nano DNA Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq Nano DNA Library Prep Kit enables preparation of uniquely indexed DNA libraries for single end and paired end sequencing.
Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin
Automated DNA Library Construction using the NEBNext® Ultra II DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith, M.S. \| Beckman Coulter, Inc. Daniela Munafo, Ph.D. \| New England Biolabs, Inc. Brad Langhorst, Ph.D. \| New England Biolabs, Inc. Laurie Mazzola \| New England Biolabs, Inc. Joanna Bybee \| New England Biolabs, Inc. Danielle Rivizzigno \| New England Biolabs, Inc. \| Published 2016
Techniques of the Coulter Principle and Dynamic Light Scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Liposomes are special lipid vesicles ranging from 50 nm to 5 μm in diameter
How to Characterize Multimodal Samples using Particle Characterization Equipment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note will discuss the topic of size resolution in particle characterization and advantages and disadvantages of various techniques
Automated Illumina TruSeq Custom Amplicon Low Input Library Preparation Kit on the Beckman Coulter Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. \| Beckman Coulter, Inc. \| Published 2016
Automated Sample Preparation for Cell-Based Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The automation of sample preparation and analysis workflows for a variety of common cellular assays.
Using Flow Cytometry to Assess Protein Aggregation in Bacterial Overexpression Systems Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes the use of Proteostat dye in a flow cytometric assay for detection of aggregated protein in bacteria inclusion bodies. This assay can be used to assess bacterial culture conditions that favor production of soluble protein. This method reduces the amount of sample preparation required compared to gel electrophoresis-based methods.
Biomek i-Series Automated Beckman Coulter Agencourt RNAdvance Tissue Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Tissue Kit uses Beckman Coulter’s patented Agencourt SPRI paramagnetic bead-based technology to isolate total RNA (microRNA and mRNA).
Evaluation of an Automated Instrument for Viability and Concentration Measurements of Cryopreserved Hematopoietic Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: S. E. SZABO, S. L. MONROE, S. FIORINO, J. BITZAN, K. LOPER \| The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Hospital, Baltimore, Maryland; Beckman Coulter Miami, Florida, USA \| Published 2004
Particle Characterization - Flow Pressurization Eliminates Bubbles for More Accuracy {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Bubbles create major issues when characterizing nanoparticles in solution because the characterization techniques typically utilize light scattering specifically when determining size distribution and stability
Cell Separation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	During various stages of the cell culturing process, centrifugation is frequently used to isolate extracellular products or to separate cells from their aqueous environment
Biomek i-Series Automated Illumina® Nextera DNA Flex Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina Nextera DNA Flex Library Prep Kit is used to prepare up to 96 indexed paired-end libraries for sequencing on Illumina platforms.
Illumina TruSeq Nano DNA Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq Nano DNA Library Prep Kit enables preparation of uniquely indexed DNA libraries for single end and paired end sequencing
Biomek i-Series Automated IDT® xGen Hybridization Capture of DNA libraries on Biomek i7 Hybrid Genomics Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Next Generation Sequencing (NGS) has enabled collection of exome-wide information faster than any previous technology.
Automated TruSeq RNA Sample Preparation from FFPE Tissue Specimens Utilizing the Biomek FXp Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Bee Na Lee Ph.D., Senior Application Scientist, and Sarah Simons, Advanced Specialist- Field Systems Support, Beckman Coulter Life Sciences \| Michaela Bowden Ph.D., Research Scientist \| Ashley Rossi, Molecular Technician, and Ed Stack Ph.D., Associate Director Center for Molecular Oncologic Pathology, Medical Oncology at DFCI \| Svitlana Tyekucheva, Department of Biostatistics, DFCI Biostatistics & Computational Biology and Massimo Loda MD., Director Center for Molecular Oncologic Pathology, Medical Oncology at DFCI and Department of Pathology at Brigham and Women's Hospital
The New Avanti J-15 Centrifuge Time Saving Deceleration Profile Improves Workflow Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The implementation of Ultra Harmonic Technology into the Avanti J-15 series renders our centrifuge ideal for applications that require multiple runs. It saves up to 10% of instrument usage time for a single application and the time savings maximizes overall laboratory assent management.
Automated Solutions for Antibody Development Using Single B Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Solutions for automating Single B Cell selection that can increase your capacity and allow you to run multiple projects in parallel, thereby reducing project cycle time while also improving quality
Automated Salt-Assisted Liquid-Liquid Extraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski and Ruth Zhang \| Beckman Coulter Life Sciences, Indianapolis, IN, USA, Yun Yun Zou and Michael Jarvis \| SCIEX, Concord, ON, Canada
A highly consistent Bradford assay on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Bradford protein assay is a simple, fast and sensitive method that is used to measure the concentration of total protein in a sample
Using the DelsaMax CORE for Molecular Weight Analysis - Protein Sizing Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Accurate determination of protein and antibody molecular weight has great impor tance in the field of molecular biology
Automated Optimization of Murine Embryonic Stem Cell Differentiation into Cardiomyocytes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski Ph.D., Li Liu Ph.D., Amy Yoder, and Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter, Inc. Indianapolis, IN \| Published 2011
A Path to the Standardization of Exosome Isolation and NGS Characterization for Complex Disease Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: CHAD SCHWARTZ, PH.D. \| ZACH SMITH, M.S. \| Published October 2015
Automation of Cell Staining Using the Biomek 4000 Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Manual cell staining of cardiomyocytes, either to characterize antigen expression or to assess cell health, is labor-intensive and time-consuming.
Biomek i-Series Automated Beckman Coulter Agencourt RNAdvance Blood Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Blood Kit uses Beckman Coulter’s patented Agencourt SPRI paramagnetic bead-based technology o isolate total RNA (microRNA and mRNA) from PAXgene preserved blood.
Challenges in Next Gen Sequencing and How to Overcome Them {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The exponential acceleration in our capacity to sequence 3.2 billion human base pairs has transformed our approach to therapeutics, particularly for cancer.
Flexible ELISA automation with the Biomek i5 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Enzyme-linked immunosorbent assays (ELISAs) are a common analytical tool for detecting the presence of a protein or other molecules of interest
Cell Culture Monitoring with the Vi-CELL MetaFLEX Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note that details how the Vi-CELL MetaFLEX is an effective tool for analyzing mammalian and insect cell culture media.
The MD Anderson SPRI8 Experience: Using Agencourt8 CleanSEQ8 and Agencourt AMPure8 in HLA Sequencing-Based Typing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Cano, Pedro \| Fernandez-Vina, Marcelo \| Guerrero, Edward \| Barnes, Titus \| Partlow, David \| Willis, Dana \| Zhao, Weicheng \| Patel, Hemantkumar \| Heins-Israel, Karen \| EL-Fahmawi, Bassam
Q&A on Detecting Moisture in Hydraulic Fluid, Oil and Fuels {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Monitoring the amount of moisture in petroleum based fluids before 100% saturation occurs will reduce degradation and will increase the life and performance of equipment components. Advancements in technology allowed Beckman Coulter to add a moisture sensor to our HIAC PODS+ Portable Liquid Particle Counters. This application note details the moisture detection application in hydraulic fluid, oil and fuels.
FormaPure DNA Extraction kit for FFPE Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The FormaPure DNA extraction kit provides a convenient extraction process utilizing Beckman Coulter’s proprietary solid phase reversible immobilization (SPRI) chemistry to extract DNA from FFPE curls.
Biomek i-Series Automation of the Apostle MiniMax™ High Efficiency cfDNA Isolation Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Apostle MiniMax™ High Efficiency cfDNA Isolation Kit isolates cell free DNA (cfDNA) and circulating tumor DNA (ctDNA) from plasma collected from blood collection tubes containing EDTA and other blood collection tube types as well as from serum and urine.
Rapid Determination of Spectrophotometric Absorptivity by Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: R. A. Gray, A. Stern, T. Bewley, and S. J. Shire Genentech, Inc. \| South San Francisco, CA
Evaluating Contact Lens Disinfection Efficacy Using the Beckman Coulter™ Multisizer™ 3 COULTER COUNTER® {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The percentage of aggregates in bacteria cultures may be determined by employing the Coulter Principle, also known as Electrical Sensing Zone (ESZ) technique
Illumina TruSight Oncology 500 Automated on the Biomek NXP Span-8 Genomics Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The TruSight Oncology 500 assay is a comprehensive next-generation sequencing (NGS) assay targeting the full coding regions of 523 genes implicated in the pathogenesis of solid tumors.
Flow Cytometric Quantification to Assess Doral Endocytosis Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of flow cytometry to examine the phagocytosis of collagen type I-coated fluorescent microspheres by rat embryonic fibroblasts. Cellular uptake can be assessed quickly in a large number of cells using flow cytometry.
Vi-CELL BLU FAST Mode Option {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note explains the optional FAST Mode of operation in the new Vi-CELL BLU which can be used in either plate or carousel configuration to significantly reduce sample to sample operation times.
Eight Color Immunophenotyping Unlysed Whole Blood Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This whole blood assay does not involve harsh lysis or centrifugation steps, minimizing any cell perturbations from sample handling. This 8-color panel provides a broad survey of peripheral blood leukocytes.
Calibration {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The calibration of a Total Organic Carbon (TOC) Analyzer is a crucial part of ensuring a quality measurement
Linearity of BSA Using Absorbance & Interference Optics {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dean Clodfelter, M.S., \|Chad Schwartz, Ph.D., Beckman Coulter Inc., 5350 Lakeview Parkway Dr., Indianapolis, IN 46268
21 CFR Part 11 Data Integrity for On-line WFI Instruments	This whitepaper takes a look at how quality-critical on-line Total Organic Carbon (TOC) and conductivity instrumentation for WFI (water for injection) quality control can be configured to help companies comply with the FDA’s expectations on data integrity, including the use of Microsoft Active Directory and PDF data export.
Thirteen Color Immunophenotyping T Cell Subsets in Whole Blood Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Contains a comprehensive overview of T cell biology and the implications for multicolor immunophenotyping panel design. Includes a detailed gating strategy for deep immunophenotyping of T cell populations including rare cell types. Also includes step-by-step instructions for setting up the CytoFLEX for multicolor immunophenotyping including use of the Compensation Library.
Exosome-Depleted FBS Using Beckman Coulter Centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D. \| Beckman Coulter Life Sciences, Indianapolis, IN
HIAC 8011+ Web Server Application Setup Procedure {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Application Scientist for Beckman Coulter Life Sciences Company in Grants Pass, Oregon, USA.
Biomek i-Series Automated Illumina TruSeq® DNA PCR-Free Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq® DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
Automation of Multi-Day Cellular Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we demonstrate the use of SAMI Process Management software to schedule and execute the automated liquid handling steps on a Biomek SAMI EX Workstation, for overlapping 8-day embryonic stem cell differentiation experiments.
21CFR part 11 Data Integrity for On-line WFI Instruments	Whitepaper by author Tony Harrison, a Compliance and Applications Specialist for Beckman Coulter Life Sciences \| Published 2017
centrifuge-whitepaer-sample-concentration-auc
Automating Biopharma Quality Control to Reduce Costs and Improve Data Integrity	This whitepaper takes a look at four common quality control procedures and how automation can help improve data integrity by reducing opportunities for human error, whilst also saving time and reducing operating costs.
Avoid the Pitfalls when Automating Cell Viability Counting for Biopharmaceutical Quality Control	In this paper we show one example of how identifying viable cells by minimum diameter alone can introduce variability of circa 33% in the reported cell concentration. This paper describes how to avoid these common pitfalls when user is adopting a new automated method of cell counting.
centrifuge-whitepaper-analysis-of-protein-protein-interactions-by-auc
Quality Control Electronic Records for 21 CFR part 11 Compliance	This paper describes how Quality Control instruments can be optimised for pharmaceutical use, helping to improve the integrity of the data in the final electronic record.
AUC INSIGHTS_Accessing the quality of AAV_Mar2020
Specifying Non-Viable Particle Monitoring for Aseptic Processing	This paper details key considerations when implementing a continuous non-viable particle monitoring (NVP) system.
The Basics and Vision of Exosome Research	Authors: Dr. Takahiro Ochiya and Dr. Yusuke Yoshioka \| Published 2016
Exosome Subclass Analysis	Authors: Dr. Kiyotaka Shiba, Dr. Satoshi Yamamoto and Dr. Kazuya Iwai \| Tokyo Japan
Centrifuge-application-Nakagomi-Fundamentals-of-Ultracentrifugal-VirusPurification
Cell Line Development – Limiting Dilution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note we describe the automation of limiting dilution on a Biomek i7 liquid handling workstation that overcomes a number of challenges associated with this workflow
Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes results obtained using a liquid handling-based automation system for the sample preparation of compounds to be analyzed by LC-MS/MS and applied to the monitoring of multiple classes of pharmaceutical and illicit drugs
Automated TruSeq RNA Sample Preparation from FFPE tissue specimens utilizing the Biomek FXp Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes automated RNA library construction using the Beckman Coulter Biomek FXP Liquid Handler from archival FFPE tissue specimens.
Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this case study, using the surface markers Ly6C (hematopoietic precursor cells, monocytes), Ly6G (granulocytes/neutrophils), and CD115 (macrophage colony-stimulating factor [M-CSF] receptor; macrophages), we examined and identified the differences in circulating cell populations in fresh blood samples.
Automation of RNA Isolation from Blood Samples stabilized in RNAgard® Blood Tubes, using the Magnetic Bead-Based Agencourt RNAdvance Blood System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Clinical Research studies often require blood sample collection at multiple geographic sites under a wide range of conditions
Getting Started with Kaluza: Loading Files and Creating Plots {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Learn how to open Flow Cytometry Standard (FCS) files in Kaluza and how to start building your own gating hierarchy using basic plot types such as histograms and biaxial plots
Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Three-dimensional (3D) cell cultures offer greater physiological relevance than monolayer cultures for cellular interaction studies and compound screens
Automated Size Selection using SPRIselect beads and the Biomek FX Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes the automation of the Size Selection using SPRIselect beads on the Biomek FX Liquid Handler.
Contamination Monitoring in the Hydraulics Industry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Dave Dunham \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
PerFix-nc protocol for PBMC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	PerFix-nc protocol for PBMC
New HIAC PODS+ …Have Internal Pump, Will Travel! {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
Whole Genome Sequencing of Microbial Communities for Scaling Microbiome and Metagenomic Studies Using the Echo 525 Liquid Handler and CosmosID {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: We demonstrate that the Echo 525 Liquid Handler can be used to miniaturize WGS of microbial communities.
Optimizing the Multisizer 4e Coulter Counter for use with Small Apertures {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The purpose of this application note is to provide some guidelines to facilitate successful testing with the Multisizer 4e when employing 30 µm and smaller.
dsDNA Quantification with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: The Echo 525 Liquid Handler was used to miniaturize and optimize fluorescent-based quantification assays.
Flexible ELISA Automation with the Biomek i5 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Automated ELISA assay workflow \| Optimized liquid transfers eliminate the need for a plate washer \| High-capacity deck accommodates fresh tip usage
Internal results based on Normal whole blood {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Internal results based on Normal whole blood
Automating Bradford Assays—Reliable Results with Less Effort {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this work we demonstrate the automated preparation and analysis of Bradford protein assay samples using a Biomek NXP Workstation with an integrated EMax® Plus Microplate Reader.
Detection Limit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	When considering which instrument is appropriate for a given application, it is common to consider various performance characteristics
Using Laser Diffraction Analysis in Pigment Sizing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Pigments and paints are an important class of industrial materials
Red Blood Cell Microparticle Analysis by Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Discover how to stain microparticles for flow cytometric analysis. The assay describes sample preparation, staining, and gating steps to distinguish red blood cell-derived microparticles from microparticles from other tissue origins. Representative data from several replicates are provided in order to report out precision obtainable from this approach.
Cell Line Development – Hit Picking {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The basic concept of hit picking is simple – 1) identify hits based on assay data and 2) consolidate these hits into a new labware in an identifiable way
Automated Cell Culture: Improving the Consistency of Cell Maintenance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we illustrate the capabilities for automating a standard workflow for cell line maintenance and demonstrate this process using mouse embryonic stem cells.
Biomek i-Series Automated Illumina TruSeq® Stranded Total RNA Sample Preparation Kit Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq® Stranded Total RNA Sample Preparation Kit protocol converts total RNA into a template library of known strand orientation (Illumina TruSeq Stranded Total RNA Library Prep Guide)
Biomek i–Series Automated Illumina® TruSight Tumor 170 32 Sample Method {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The TruSight® Tumor 170 sequencing assay is a qualitative oncology test optimized for the detection of gene variants across 170 gene targets in nucleic acids (DNA and RNA) extracted from formalin–fixed, paraffin–embedded (FFPE) cancer tissue samples.
Beer, Evaluation of Final Product and Filtration Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The concentration and size distribution of particles in beer may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method
Automating Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features automating cell line development with a Biomek i7 liquid handling workstation and integrated devices in order to overcome challenges for this workflow
Application Note: Advanced analysis of human T cell subsets {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13-color tube based on DURAClone dry reagent technology
A highly consistent BCA assay on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The BCA (bicinchoninic acid) assay is a popular method used in quantification of proteins
Biomek i-Series Automation of the Beckman Coulter GenFind V3 Blood and Serum DNA Isolation Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The GenFind V3 Blood and Serum DNA Isolation Kit from Beckman Coulter isolates high quality genomic DNA from fresh or frozen whole blood and serum containing Citrate, EDTA, or Heparin anticoagulants, as well as from cultured cells.
Assessment of T-cell receptor repertoire by flow cytometry using the Beta Mark TCR Vβ repertoire kit and Kaluza analysis software {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	T-cells play a central role in the immune system by recognizing and responding to non-self-antigens. T-cell receptors (TCRs) are located on the T-cell surface and are responsible for interacting with peptide fragments of antigens that bind to major histocompatibility complex (MHC) molecules.
Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate: Biomek Wide Bore vs. Standard Pipette Tips {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Robert D. Lund, Beckman Coulter Life Sciences, Indianapolis, IN \| Published: 2016
Minimizing process variability in the manufacturing of Bottled Drinking Water {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note by Bill Bars detailing the use of the HIAC 8011+ for variability testing of bottled drinking water.
Evaluation of Instrument to Instrument Performance of the Vi-CELL BLU Cell Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note examines the comparability of results from multiple Vi-CELL BLU cell viability analyzers.
A Highly Consistent Lowry Method on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The quantification is based on peptide bonds of proteins reacting with copper under alkaline conditions followed by copper-catalyzed oxidation of aromatic amino acids, producing blue color
Analyzing Mussel/Mollusk Propagation Using the Coulter Analyzer Multisizer 4e {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details how the use of a Coulter Principle based instrument provides a major step forward in accurately verifying both the size of the feed and concentration for a fish hatchery.
The Coulter Principle for Outlier Detection in Highly Concentrated Solutions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Particle characterization techniques generally fall into one of two categories: “ensemble” techniques (multiple par ticles are analyzed simultaneously), or “discrete” techniques (individual particles are measured one at a time)
Switching from Oil Testing to Water and Back using the HIAC 8011+ and PODS+ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details the processes required when switching from water based sampling to oil based sampling and/or from oil based sampling to water based sampling in the HIAC 8011+ and HIAC PODS+
ICH Q2 – the Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems	This paper discusses some of the challenges when using TOC analysers to demonstrate pharmacopoeial TOC level compliance for modern water systems in the light of the ICH Q2 document2 from the International Conference on Harmonisation.
High-Throughput Illumina® TruSeq® Nano DNA Library Construction on the Biomek FXP Dual-Arm Multi-96 and Span-8 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Mary Blair
Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The introduction of exogenous nucleic acids into cells is an essential means of studying cell biology.
Automated XTT Assay for Cell Viability Analysis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cellular viability assays are valuable for numerous workflows—from establishing proliferation rates to drug toxicity screens.
Biomek i-Series Automation of the Beckman Coulter Agencourt DNAdvance Genomic DNA isolation Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt DNAdvance DNA isolation kit uses Beckman Coulter’s patented Agencourt SPRI paramagnetic bead based technology to isolate genomic DNA from a variety of sources.
Biomek i-Series Automated Beckman Coulter Agencourt SPRIselect for DNA Size Selection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The library preparation process for Next Generation Sequencing requires a uniform distribution of nucleic acid fragments within a specific size range.
Real Time Determination Of Cell Volume Changes Of Sperm Used For Cryopreservation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Much of the cellular damage produced by freezing is due to freezing-induced dehydration and thawing-induced rehydration.
Application note: Advanced analysis of data obtained with DuraClone RE CLB {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note for advanced analysis of data obtained with DuraClone RE CLB
Using Advanced Plot Types in Kaluza: Comparison Plots for Visualization of the TCR Vbeta Repertoire {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Introduces the Comparison Plot as a tool to compare several data sets in line or bar charts. See how Comparison Plots can be used to visualize a clonogram representation of the TCR Vβ repertoire.
What to do now that ACFTD is Discontinued {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note will examine the steps that optical Particle Counter users need to take to transition from an ACFTD calibration to an ISO-MTD calibration, the modern equivalent.
Ten Color Immunophenotyping of Mouse NK Cells Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Surface-marker expression is critical to the delineation of NK cell maturity, function, and localization. We present a protocol and gating strategy for the identification of splenic murine NK cell populations via the CytoFLEX flow cytometer using a 10-color screen.
High Purity Simultaneous Cell Sorting of Nano and Large Phytoplankton Using the MoFlo Astrios EQ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Phytoplankton conversion of light on the upper limits of the ocean consists of half of the photosynthesis on the Earth
ZAP-70 assessment by flow Cytometry: improving assay sensitivity, robustness and workflow using an isoclonic control and PerFix-nc. {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ZAP-70 assessment by flow Cytometry: improving assay sensitivity, robustness and workflow using an isoclonic control and PerFix-nc.
Stem Cell Analyses Using The Vi-CELL™ XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Stephen E. Szabo, Ph.D. \| Sarah Monroe, B.S.
Analysis of Membrane Protein Dimerization by Sedimentation Equilibrium {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Karen G. Fleming - Department of Molecular Biophysics and Biochemistry, New Haven, CT
Viral RNA Extraction Performance using RNAdvance Viral Supplemental Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note we present a research study at Miami Cancer Institute in collaboration with Beckman Coulter Life Sciences using the RNAdvance Viral research reagents described in the Viral Nucleic Acid extraction from swabs using RNAdvance Viral supplemental protocol.
Vi–CELLBLU im Fast Mode {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Vi–CELL BLU mit einem optionalen Betriebsmodus „Schnell“ ausgestattet, der in Platten- wie auch Karussellkonfi gur ationen genutzt werden kann, um den Wechsel von einer Probe zur nächsten erheblich zu beschleunigen
Application Note: Compensation setup for high-content DURAClone reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Compensation setup for high-content DURAClone reagents
USP System Suitability {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The US Pharmacopeia (USP 36-NF 31, section <643>) provides specific guidance on how to qualify TOC instrumentation for use
Illumina TruSeq Exome Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq Exome Library Prep kit is a workflow to generate indexed paired-end libraries and is specially designed to work with Formalin Fixed Paraffin Embedded (FFPE) samples
Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph.D., Dean Clodfelter, M.S. \| Beckman Coulter, Inc., Indianapolis, IN 46268
Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi-Cell™ XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Stephen E. Szabo, Ph.D. \| Sarah Monroe, B.S. \| Beckman Coulter, Inc. \| Published 2004
Secondary Screening Solutions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For drug discovery and biomarker research, our series of Biomek automated workstations provide comprehensive tailored solutions for your high throughput screening workflows
Automated Liquid Handling for Cell Staining and Imaging {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist \| Li Liu, Sr. Development Scientist \| Amy Yoder, Staff Development Scientist \| Beckman Coulter Life Sciences Indianapolis, IN
Biomek FXP Laboratory Automation Workstation as a Tool for Discovering Targets & Compounds for Host-Directed Influenza Therapy {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Markus Lesch, Project Manager, Center for Systems Biomedicine, Steinbeis Innovation gGmbH. Falkensee, Germany, Alexander Karlas, Senior Scientist, Max Planck Institute for Infection Biology, Berlin, Germany. Published: 2015
HIAC PODS+ Reporting Standards Synopsis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
Automated salt-assisted liquid-liquid extraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this work, we have utilized a Biomek NXP Workstation to automate the liquid handling steps of salt-assisted liquid-liquid extraction of 25-OH-Vitamin D from serum
Automated Sample Preparation for Analysis of 25-OH Vitamin D3 and 25-OH-Vitamin D2 by LC/MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this work, we demonstrate the feasibility of automating the sample preparation for liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis of 25-OH-Vitamin D2 and 25-OH-Vitamin D3.
High Throughput Screening {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For drug discovery and biomarker research, screening campaigns are employed to identify potential new treatments for diseases and to answer questions that remain unknown in the scientific community.
Automated Cell Transfection and Reporter Gene Assay {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We demonstrate here how a reporter gene assay can be automated using two Biomek NXP workstations, yielding accurate and highly reproducible measurements of reporter activity
Biomek Automated 384-well qPCR Setup using KAPA Biosystems Library Quantification Kit for Illumina Sequencing Platforms {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Library quantification is an essential step in the illumina next-generation sequencing(NGS) workflow
Simple, Yet Effective – Automating Bradford Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Excellent consistency of automated standard dilution and Bradford assay preparation
Automated Solutions for Hybridoma Antibody Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Solutions for automating the critical steps of hybridoma development that increase productivity and make it possible to produce specific monoclonal antibodies
Miniaturized Multi-Piece DNA Assembly Using the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In the studies described in this Application Note, the NEB NEBuilder HiFi DNA Assembly Cloning Kit was combined with the Echo 525 Liquid Handler to assemble a construct over five modular pieces
Reproducible and Efficient Automation of DTR Purification Using the Biomek 4000 Workstation and CleanSEQ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt CleanSEQ* application for the Biomek 4000 Laboratory Automation Workstation is a rapid, high performance dye-terminator removal method utilizing Solid Phase Reversible Immobilization (SPRI) paramagnetic beadbased technology.
Use Machine Learning Algorithms to Explore the Potential of Your High Dimensional Flow Cytometry Data Example of a 20–color Panel on CytoFLEX LX {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Understand how to perform machine learning algorithms like viSNE and FlowSOM to identify phenotypes of populations/subsets present in the 20–color CytoFLEX LX flow cytometry data
Root cause investigation for pharmaceutical water systems	In this whitepaper we discuss some typical sources of contamination in water systems and how root-cause investigations can be supported using on-line Total Organic Carbon (TOC) analysers.
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
Preparing Reagent Solution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The QbD1200 uses a mixture of phosphoric acid (to aid in removal of CO2 from solution) and ammonium persulfate (to aid in oxidizing organic compounds)
Evaluation of an Automated Instrument for Viability and Concentration Measurements of Cryopreserved Hematopoietic Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Two important parameters for determination of deleterious effects of cellular processing on hematopoietic progenitor cells are cell viability and concentration. The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins Hospital evaluated the Beckman Coulter Vi-Cell automated instrument for the measurement of these two parameters.
Inorganic Carbon Removal {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	A water sample initially contains two types of carbon: Total Inorganic Carbon (TIC) (from CO2 gas dissolved in H2O and dissolved carbonates in the water) and Total Organic Carbon (TOC) (from organic species)
Cell Line Development – Hit Picking {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note we discuss how hit picking was automated as part of a cell line development workflow
Enhanced Forward Scatter Module+ on the MoFlo™ Astrios: Small Particle Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr. Carley D. Ross, Alan Dean Ph.D., Kerry Weinberg, Robin Morris, Blake McCarty and Nicole Lewis \| Beckman Coulter, Inc., Flow Cytometry Business Center, Fort Collins, CO, USA
cell-viability-vi-cell-blu-match-cell-counts-xr-blu-201904-german {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Vaporized Hydrogen Peroxide Decontamination of Vi-CELL BLU Instrument {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the method and results for testing the Vi-CELL BLU for VHP resistance.
Continuous Cell Culture {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Continuous cell culture provides scientists the ability to readily have assay-ready plates available to test potential agents or to have large quantities of multiple cell lines available for therapies research.
Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As biologics research continues to evolve and gain prominence in therapy development for disease states, the need for reliable and robust cell lines will greatly increase
Advancing Science is in our DNA {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	From the Human Genome Project to today’s quest for precision medicine, we’ve long invested in providing genomic products that reduce complexity and improve productivity
Flow Cytometric Analysis of Auto-fluorescent Cells in Marine Demosponge Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Natural autofluorescence of marine organisms can be detected in flow cytometers. Utilizing this, an assay to assess aggregation and reorganization of the sponge is described.
CD6: New Low/Negative Surface Marker for Human FOXP3+ Naturally-occurring Regulatory T-cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Carlos A. Garcia Santana M.D., Ph.D. \| James W. Tung Ph.D. \| Sergei Gulnik Ph.D.
Competitive Mouse Bone Marrow Transplantation Reconstitution Assay Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Using primary-conjugated antibodies against CD45.1 and CD45.2, we measure competitive bone marrow transplantation success by quantifying the proportion of donor cells (marked by CD45.1/Ly5.1 expression) and recipient cells (marked by CD45.2/Ly5.2 expression) in the peripheral blood of irradiated recipient mice post-transplantation.
Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DURAClone dry reagent technology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DURAClone dry reagent technology
Method for Determining Cell Type Parameter Adjustment to Match Legacy Vi-CELL XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note assists in matching Vi-CELL BLU cell types to give cell counts and viability levels equivalent to those obtained on existing Vi-CELL XR instruments.
Density Gradient Separation of Gold Nanorods - Using the New Avanti JXN Centrifuge & JS-24.15 Swinging Bucket Rotor {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Gold Nanorods (AuNR) hold great promise for biomedical imaging
A Fully-Automated, Image-Based Screen for miRNAs that Regulate Cancer Cell Viability and Chemosensitivity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist, \| Beckman Coulter Life Sciences, Indianapolis, IN \| Tammy Chan, Field Application Scientist, \| Molecular Devices, Sunnyvale, CA \| Kristin Prasauckas, Manager, Field Applications Scientist, \| Molecular Devices, Sunnyvale, CA \|Tara Jones-Roe, Marketing Manager, \| Beckman Coulter Life Sciences, Indianapolis, IN
Identification of Natural Killer Cells in Rat Placenta Model of Preeclampsia Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	A special population of natural killer cells (uNK) has been implicated in preeclampsia. uNK cells have contact with the trophoblast, modify uterine arteries to promote blood flow, and modulate trophoblast invasion in a dose-dependent manner. In this application note a detailed method for preparing resident NK cells from tissue and an assay to access NK cell activation is provided.
The Solution Conformation of Novel Antibody Fragments Studied Using the Proteomelab XL –A Analytical Ultracentrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Peter J. Morgan, Olwyn D. Byron, Stephen E. Harding \| Department of Applied Biochemistry and Food Science \| University of Nottingham, Sutton Bonington, U. K.
Mo Bio PowerMag Soil DNA Isolation Kit on Biomek 4000 Liquid Handling Automation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Shilpa Parakh, Beckman Coulter, Inc \| Published 2016
The Sizing of Non-Spherical, Sub-Micron Particles using Polarization Intensity Differential Scattering (PIDS™) {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nearly all laser-based particle-sizing devices make no allowance for the shape of the materials under test, regardless of the size of the particles
Industrial Application Note 8011+ Reporting Standards Feature and Synopsis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Application Scientist for Beckman Coulter Life Sciences Company in Grants Pass, Oregon, USA.
Automating 3-D Cell Culture and Screening by Flow Cytometry and High-Content Imaging {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Michael Kowalski, Ph.D. \| Staff applications scientist for Beckman Coulter Life Sciences Indianapolis, IN \| Published 2016
Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note about the use of the Vi-CELL MetaFLEX for detecting small changes in metabolic activity in different cell types.
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek NXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Amy Yoder, Michael Kowalski Ph.D., Li Liu Ph.D., Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter Inc., Indianapolis, IN \| Published 2011
ISO11171-2010 Standard Requirement Changes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Applications Scientist for Beckman Coulter, Inc. in Grants Pass, Oregon, USA
Rapid and Easy Intracellular + Surface Staining of MOUSE Cells using PerFix-nc {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Dispense 50 μL of cells into empty tube \| Add 5 μL of fixative reagent R1 (low fixation), mix tube \| Incubate 15 min. at room temperature (RT)
Custom Design Service Reagents: Recommendations on Lysis buffers and Protocols {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Custom Design Service Reagents: Recommendations on Lysis buffers and Protocols
Full Automation of the SISCAPA® Workflow Using a Biomek NXP Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Morty Razavi, Michael Kowalski, Tara Jones-Roe, Christie Hunter, Leigh Anderson, Richard Yip, Matt Pope, Terry Pearson SISCAPA Assay Technologies, Washington DC
Considerations of Cell Counting Analysis when using Different Types of Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note reviews several different cell types and cell preparation approaches using standard cells.
Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe the automation of the New England Biolabs NEBNext (Registered trademark) Ultra Directional RNA Library Prep Kit for Illumina on the Beckman Coulter Biomek FXP automated liquid handler.
Enumeration and Size Distribution of Yeast Cells in the Brewing Industry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Steve Szabo, Ken Lindo, Brendan Yee \| Cellular Analysis Business Center, Beckman Coulter Inc, Miami, FL
使用超速离心机进行腺相关病毒的分离纯化 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	使用超速离心机进行腺相关病毒的分离纯化
Kaluzaにおける高度なプロットの使用 - 比較プロットを使用したTCR Vβレパトワの可視化 - {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	折れ線グラフまたは棒グラフで複数のデータセットを比較するツールとして比較プロット（Comparison Plot）を紹介します。比較プロットを使用してTCR Vβレパトワの発現を視覚化する方法とKaluza Analysis ソフトウエアを使用して比較プロットを作成する方法を紹介します。
フローサイトメトリーによる簡単な17 マーカー、 18カラーのヒト血液フェノタイピング {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	CytoFLEXとDURACloneを用いて18カラーパネルを迅速にデザインし、テストを行う方法を説明します。
Applcation:超遠心分析を用いたバイオ医薬品インスリンのキャラクタリゼーション {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	、製剤処方検討での幅広い条件下でバイオ医薬品の評価に適用可能なBeckman Coulter 社の分析用超遠心機 ProteomeLab XL-I が有効に利用された例を紹介します。
ヒト血清アルブミン-ポルフィリン複合体の多波長超遠心分析 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	超遠心分析（Analytical Ultracentrifugation, AUC）は、生理学的な溶液の条件下でコロイド系の物理化学的な性状を決定するための方法です。
レンチウイルス・ベクターの調製法（アプリケーションノート） {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	超遠心機Optima X シリーズおよびスウィングロータSW 32 Ti を使用したレンチウイルス・ベクターの調製法
アプリケーションノート：Vi-CELL XRを使った生死細胞数測定の自動化 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Vi-CELL XRは細胞の計数、生存率などの解析を自動化します。トリパンブルー染色法にて、画像解析によるセルカウンターです。人為的な誤差や、細胞に合わせたセルカウントができます。
アプリケーションノート：良好な粒度分布結果を得るためのサンプル前処理方法について {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	粒子サンプルの正確な測定のために、重要な要素が測定前のサンプル調製。レーザー回折・散乱法は、測定方法に湿式測定と乾式測定があり、測定方式により分散方法も異なる。本稿では、この各種の分散方法を紹介。
アプリケーションノート：動的光散乱法では再現性が悪く、不安定で困っていませんか {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ベックマン・コールター社のレーザー回折・散乱法を原理とした粒度分布測定装置（LS13320XR)用いて、これまで難しいとされた多分散系ナノ粒子を動的光散乱法（DLS)並みに測定できることを確認
アプリケーションノート：粒子物性装置を使用してマルチモーダルサンプルを用いた特性評価 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	粒度分布測定における粒子径分解能や、各原理における長所と短所について説明。複数の原理の異なる粒子径測定機器を用いて、複雑な多分散系の混合サンプルを用いて測定した事例
アプリケーションノート：LS13 320 XRは10 nm-2000µmまで死角なし {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ベックマン・コールター社のレーザー回折・散乱法を原理とした粒度分布測定装置（LS13320XR)用いて、ナノ粒子から大粒径サイズの粒子までの多種の標準粒子を混合した多分散系サンプルを測定
アプリケーションノート：ファインバブルのサイズと粒子数を正確に測定 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ファインバブル（マイクロバブル・ナノバブル）は、環境や医療の分野で、水質浄化や殺菌等への実用化を目指している粒子の一つ。このファインバブルをコールターカウンター（Multisizer4e）で測定。
アプリケーションノート：産業用ドライカーボンの粒度分布測定 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ベックマン・コールター社のレーザー回折・散乱法を原理とした粒度分布測定装置（LS13320XR)と、他社製同原理の粒度分布測定装置を用いて、産業用ドライカーボンを測定比較
アプリケーションノート集フローサイト基礎の基礎 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	フローサイトメーターをこれから使っていこうとお考えの方を対象に、「今さら聞けないフローサイトメトリーの基礎」と題し、基礎的な内容をご紹介いたします。
ナノテクノロジーアプリケーションノート集 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ベックマン・コールター社製品である遠心機、粒子計測装置、自動分注機を用いたナノ粒子サンプルの測定事例集。インク、金属コロイド、ナノセルロース、カーボンナノチューブなど、幅広いサンプルについて紹介
アプリケーションノート：細胞代謝活性モニタリングにおけるVi-CELL MetaFLEXの有用性 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	様々な細胞の培養が可能になり、その性状解析の重要性が増しています。Vi-CELL MetaFLEXによる培地分析から、細胞の代謝をモニタリングすることで、培地の調製や代謝活性を評価することができます。
アプリケーションノート：高速培地環境分析装置 Vi-CELL MetaFLEXによる細胞培養モニタリング {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Vi-CELL MetaFLEXはバイオロジクスの品質を決める培養細胞の培地分析装置です。MetaFLEXに搭載されているセンサー技術ならびに測定精度、さらに機器間差について説明します。
アプリケーションノート:生死細胞オートアナライザー Vi–Cell BLU の計数性能評価 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	セルカウンターとしての性能を評価するため、NISTで採用されている方法を用いて細胞の低濃度から高濃度に至る広い濃度範囲を評価しました。Vi-CELL BLUは高い計数性能を有していることを示します。
アプリケーションノート：Vi-CELL XRを使った生死細胞数測定の自動化 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Vi-CELL XRは細胞の計数、生存率などの解析を自動化します。トリパンブルー染色法にて、画像解析によるセルカウンターです。人為的な誤差や、細胞に合わせたセルカウントができます。
アプリケーションノート：生死細胞オートアナライザー Vi-CELL XRによる酵母の生存率、細胞濃度測定 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Vi-CELL XRは酵母細胞のカウントも可能です。動物細胞、昆虫細胞と異なり、酵母細胞は細胞壁を有するため、酵母細胞計測のための計測条件設定を行うことで計数が可能になります。
コールターカウンター基礎講座 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	1940年代、Wallace H. Coulterが開発した粒子数と粒子サイズの測定技術の電気的検知帯法（コールター原理）を解説。さらに、粒子計測の基礎として、粒子サイズの定義や計測技術での違いも解説
アプリケーションノート：Vi–CELL BLU 高速モード {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Vi-CELL BLUに搭載されている高速モードは、セルカウントの解析速度を大きく短縮します。様々な濃度のサンプル計測においてもキャリーオーバーはほぼ影響なく、正確にセルカウントが行えます。
アプリケーションノート:Vi–CELL XRとVi–CELL BLUとの仕様比較 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	グローバルスタンダードのセルカウンターVi-CELL XRの優れた機能を継承し開発されたVi-CELL BLUの仕様について、Vi-CELL XRと比較しました。
アプリケーションノート：過酸化水素ガス滅菌によるVi–CELL BLU の性能評価 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	クリーンルームで機器を使用する場合、機器が除染できることが望まれます。クリーンルームでのVi-CELL BLUの使用を目的とし、過酸化水素ガス滅菌による機器性能への影響を評価を行いました。
アプリケーションノート：96ウェルプレートを利用して細胞の計数条件を決める有効な方法 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	セルカウントまでの細胞の準備は従来の方法で大丈夫でしょうか。Vi-CELL BLUのプレート機能を使うことで様々な細胞の新たな特徴が見えてきます。
アプリケーションノート：Vi–CELL BLU の機器間差評価テスト {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	セルカウンターをはじめ、あらゆる機器の機器間差が小さいことは、課題の一つです。GMP施設、遠隔共同研究において、Vi-CELL BLUの大きな強みは機器間差が最小限に抑えられるよう設計されています。
アプリケーションノート:ミネラルウォーター製造工程のばらつき最小化 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ミネラルウォーター製造における、ろ過効率や精製プロセスの管理のためにベックマン・コールター社製液中パーティクルカウンター（8011＋）を用いたもの。8011+で、製造～容器充填後までの粒子数管理を実現
アプリケーションノート：Vi–CELL XR とVi–CELL BLUの計数マッチング方法 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	新製品Vi-CELL BLUのセルカウントをVi-CELL XRのセルカウントと一致させる方法を紹介します。新技術を搭載したセルカウンターをいかに既存機器の計数に合わせるか詳しく説明します。
アプリケーションノート：Vi-CELL BLUの計数値をVi-CELL XRに素早く一致させるための有効な方法 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Vi-CELL BLUのプレート機能を使うことによって、Vi-CELL XRのセルカウントに素早く一致させる方法を紹介します。
MODBUS プロトコルによるHIAC PODS+ リモートコントロール {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ベックマン・コールター社の油中パーティクルカウンター（HIAC PODS+）を、Modbusユーティリティを利用してリモートコントロールするための設定を紹介
557A Multisizer 4e {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Optimizing the Multisizer 4e Coutler Counter for use with Small Apertures {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
17-Marker, 18-Color Human Blood Phenotyping Made Easy with Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Particle Size Analysis Simple, Effective and Precise {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Cell Line Development – Selection and Enrichment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Critical Particle Size Distribution for Cement using Laser Diffraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System: an application for robust and scalable cancer research and biomarker discovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
How to Use Violet Laser Side Scatter Detect Nanoparticle {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Matching Cell Counts between Vi–CELL XR and Vi–CELL BLU {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Vi CELL BLU FAST Mode Option {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Considerations of Cell Counting Analysis when using Different Types of Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Efficient Factorial Optimization of Transfection Conditions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
A Highly Consistent Lowry Method on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Cell Line Development – Hit Picking {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Particle diameter measurement of a nanoparticle composite - Using density gradient ultracentrifugation and dynamic light scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Preparation and purification of carbon nanotubes using an ultracentrifuge and automatic dispensing apparatus, and analysis using an analytical centrifuge system {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
ICH Q2 – The Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
LS 13 320 XR: Sample Preparation - How to measure success {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
JP SDBS Validation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Background Subtraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Enumeration And Size Distribution Of Yeast Cells In The Brewing Industry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automated XTT Assay for Cell Viability Analysis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Echo System-Enhanced SMART-Seq v2 for RNA Sequencing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Beer, Evaluation of Final Product and Filtration Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Miniaturization and Rapid Processing of TXTL Reactions Using Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Flexible ELISA automation with the Biomek i5 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Cell Line Development – Limiting Dilution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Cell Line Development – Data Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
USP System Suitability {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
HIAC PODS+ versus Parker ACM-20 Performance comparison {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Highly Reproducible Automated Proteomics Sample Preparation on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
A workflow for medium-throughput isolation of cfDNA from plasma samples using Apostle MiniMax™ on the KingFisher™ Technology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Achieving Compliant Batch Release – Sterile Parenteral Quality Control {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Analyzing Mussel/Mollusk Propagation using the Multisizer 4e Coulter Counter {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
21 CFR Part 11 Data Integrity for On-line WFI Instruments {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Minimal Sample to Sample Carry Over with the HIAC 8011+ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
dsDNA Quantification with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
A Highly Consistent Bradford Assay on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Isolation of cell-free DNA (cfDNA) from plasma using Apostle MiniMax™ High Efficiency cfDNA Isolation kit— comparison of fully automated, semi-automated and manual workflow processing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automated salt-assisted liquid-liquid extraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Consistent Cell Maintenance and Plating through Automation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Overload Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Inorganic Carbon Removal {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Detection Limit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Optimizing the HIAC 8011+ Particle Counter for Analyzing Viscous Fluids {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Calibrating the QbD1200 TOC Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Method Overview {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
HIAC PODS+ Online Mode & Filter Cart Mode {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automated Cell Transfection and Reporter Gene Assay {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
8011+ Reporting Standards Feature and Synopsis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Determination of Size and Concentration of Particles in Oils {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
QbD1200 Preparing Reagent Solution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Metal colloid purification and concentration using ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
A method of grading nanoparticles using ultracentrifugation in order to determine the accurate particle diameter {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Grading of pigment ink and measurement of particle diameter using ultracentrifugation / dynamic light scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Separation and purification of metal nanorods using density gradient centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Grading of nanocellulose using a centrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
A Highly Consistent BCA Assay on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Air Particle Monitoring ISO 21501-4 Impact {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Pharma Manufacturing Paperless Monitoring {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Miniaturized Sequencing Workflows for Microbiome and Metagenomic Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automating Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Avoid the Pitfalls When Automating Cell Viability Counting for Biopharmaceutical Quality Control {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Using Machine Learning Algorithms to Provide Deep Insights into Cellular Subset Composition {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Революция в аналитическом ультрацентрифугировании: новая центрифуга Optima AUC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automated Transfection Methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi‑CELL XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automated Genomic Sample Prep Ampure XP PCR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Ускорение ваших геномных исследований с автоматизацией пробоподготовки на Biomek {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automated Genomic Sample Prep Sample Quality Control {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automating Biopharma Quality Control to Reduce Costs and Improve Data Integrity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automating Cell-Based Processes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Automating Bradford Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Applications of Ultracentrifugation in Purification and Characterization of Biomolecules {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Cleanroom Routine Environmental Monitoring – FDA Guidance on 21 CFR Part 11 Data Integrity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
The new Avanti J-15 Centrifuge Improves Sample Protection and Maximizes Sample Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Conducting the ISO 14644-3 Cleanroom Recovery Test with the MET ONE 3400 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Compensation Setup For High Content DURAClone Reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
The New Avanti J-15 Centrifuge Time Saving Deceleration Profile Improves Workflow Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Efficient kit-free nucleic acid isolation uses a combination of precipitation and centrifugation separation methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Cell Counting Performance of Vi–Cell BLU Cell Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Detecting Moisture in Hydraulic Fluid, Oil and Fuels
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Biomek Automated NGS Solutions Accelerate Genomic Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Counting Efficiency: MET ONE Air Particle Counters and Compliance to ISO-21501 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Control Standards and Method Recommendations for the LS 13 320 XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Avanti JXN Protein Purification Workflow {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Changes to GMP Force Cleanroom Re-Classifications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}