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Название	Описание
CytoFLEX {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Flow Cytometric Approach to Probiotic Cell Counting and Analysis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	<p>In this paper you will:</p> <ul> <li>Compare and contrast culture-based and flow cytometry based workflows for probiotic cell enumeration</li> <li>Learn how to set up the flow cytometer for optimal detection of bacterial strains.</li> <li>See a detailed staining protocol and gating strategy for enumerating live versus dead probiotic cells.</li> </ul>
Viability Assessment of Cell Cultures Using the CytoFLEX {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
A Rapid Flow Cytometry Data Analysis Workflow Using Machine Learning- Assisted Analysis to Facilitate Identifying Treatment- Induced Changes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	<p>In this Application Note you will learn:</p> <ul> <li>How to leverage a dimensionality reduction algorithm like viSNE for visual comparisons between groups of samples</li> <li>How FlowSOM can help you in highlighting differences in abundance of specific clusters in your experimental groups</li> <li>How to use heatmaps to help you identify the phenotype of a specific cluster/metacluster</li> </ul>
Analysis of plant genome sizes using flow cytometry: a case study demonstrating dynamic range and measurement linearity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Detecting and counting bacteria with the CytoFLEX research flow cytometer: II-Characterization of a variety of gram-positive bacteria {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Flow Cytometric Analysis of auto-fluorescent cells found in the marine demosponge Clathria prolifera {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Vesicle Flow Cytometry with the CytoFLEX {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Extracellular Vesicles (EVs) are released by all cells and carry diverse cargo capable of eliciting functional changes in nearby or distant cells. Measurement of EVs and their cargo has broad diagnostic applicability.
Многоволновое аналитическое ультрацентрифугирование человеческого сывороточного альбумина, образующего комплекс с порфирином {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	<p>Аналитическое ультрацентрифугирование (AUC) – метод, который используют для характеризации коллоидных систем в физиологических условиях.</p> <p>Недавно разработанный для аналитической ультрацентрифуги Optima AUC компании Beckman Coulter многоволновой (MWL) детектор позволяет одновременно проводить гидродинамическое разделение коллоидных смесей и спектральную деконволюцию взаимодействующих и невзаимодействующих растворенных компонентов смеси. </p>
Линейность результатов изучения олигомеризации БСА методом AUC с использованием оптики поглощения и интерференции {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	<p>Аналитическая ультрацентрифуга Optima AUC — одна из последних разработок компании Beckman Coulte. Наряду с множеством усовершенствований пользовательского интерфейса на новой платформе существенно модернизированы оптическая, термическая и приводная системы. Благодаря этому обеспечивается удобство прибора для конечного пользователя с точки зрения качества данных и простоты использования.</p>
Applications of Ultracentrifugation in Purification and Characterization of Biomolecules {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Ultracentrifuges spin samples with centrifugal forces typically spanning 100,000 – 1,000,000 x g. At these high forces, the constituent molecules in the sample separate based on their physical properties (e.g., size, mass, density, anisotropy).
QbD1200 Preparing Reagent Solution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The QbD1200 TOC Analyzer uses a mixture of phosphoric acid (to aid in removal of CO2 from solution) and ammonium persulfate (to aid in oxidizing organic compounds).
Illumina Nextera Flex for Enrichment on the Biomek i7 Hybrid Genomics Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this flyer, we describe and demonstrate the automated performance of the Illumina Nextera Flex for Enrichment assay on the Biomek i7 Hybrid Genomics Workstation.
Background Subtraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	When measuring a water sample to quantify TOC (Total Organic Carbon), most TOC analyzers add some combination of reagents and dilution water to the initial sample in order to complete the measurement.
Vi CELL BLU FAST Mode Option {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper explains the optional FAST Mode of operation in the Vi-CELL BLU cell viability analyzer which can be used in either plate or carousel configuration to significantly reduce sample to sample operation times.
USP System Suitability {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	QbD1200 is designed so that System Suitability is very convenient and easy to perform.
Compensation Setup For High Content DURAClone Reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this DURAClone IM Tube application note, you will how our proprietary dry reagent formulation secures long-term fluorochrome stability while stored at room temperature.
Method Overview {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The QbD1200 is unique in that it combines all required reagents (acid, oxidizer, and dilution water) into a single reagent instead of requiring multiple reagents.
Cell Counting Performance of Vi–Cell BLU Cell Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	To determine the cell counting performance of the Vi–CELL BLU we adapted the protocol outline by NIST to determine the performance of the new Vi–Cell BLU Cell Viability Analyzer.
Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Insulin is a well characterized drug used to treat diabetes. A large number of insulin formulations have been investigated in order to develop the optimum time delivery suited to patient needs. In addition, a number of insulin analogs such as Insulin Glargine and LysPro insulin (Humalog) have been developed and marketed as derivatives for immediate use at meal time and a more sustained release.
JP SDBS Validation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The QbD1200 has a built in qualification routine, “SDBS”, which makes performing a JP 16 compliant validation convenient.
Conducting the ISO 14644-3 Cleanroom Recovery Test with the MET ONE 3400 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note reviews the test method and provides guidance on selecting both the “Challenge” and the “Target Cleanliness Limit”. Attention is given to the requirement that the test concentration should not be so high that “residue contamination” becomes a risk.
How Violet Side Scatter Enables Nanoparticle Detection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Explore how violet side-scatter enabled nanoparticle detection at 80 nanometers and above on the CytoFLEX Platform of Flow Cytometers.
Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	AUC provides a matrix-free environment that allows the near-native state characterization of a wide range of molecules. Our recently launched Optima AUC is the only commercially available instrument equipped with a state of the art optical system.
HIAC Industrial – Our overview solution for fluid power testing for all applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details measuring fluid contamination by cleanliness standards like ISO 4406, SAE AS4059, and NAS 1638 for oil and hydraulic fluid applications.
Cleanroom Routine Environmental Monitoring – FDA Guidance on 21 CFR Part 11 Data Integrity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper examines the risk of error with SOPs being implemented manually. Technology exists and is commercial available to mitigate this problem and make programs more robust, reducing the impact on data integrity and also supporting the industry's move towards environmental monitoring by production staff in the cleanroom.
Counting Efficiency: MET ONE Air Particle Counters and Compliance to ISO-21501 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The one parameter that stands alone as the champion of confusion for defining air particle counter performance is counting efficiency. The purpose of this paper is to present counting efficiency in terms that are easy to understand.
A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System: an application for robust and scalable cancer research and biomarker discovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of FormaPure XL Total, a total nucleic acid extraction kit for FFPE samples, in conjunction with the KingFisher™ Duo Prime Sample Purification System as a potential solution that mitigates some of the challenges with FFPE workflows.
Efficient kit-free nucleic acid isolation uses a combination of precipitation and centrifugation separation methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Immunotherapy and gene therapy requires highly purified DNA to deliver a gene editing message, or plasmid, to the host cell. Kit free plasmid miniprep has been traditionally used to deliver high quality and high yield DNA. This method is accomplished by a combination of salts and isopropanol and the efficient precipitation of nucleic acids from solutions via centrifugation. It is high-throughput, cost efficient and it produces higher yields as there is no loss of material due to membrane or filter interaction.
Biomek i-Series Automated Illumina TruSeq® Stranded Total RNA Sample Preparation Kit Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this technical note, we automate the Illumina TruSeq Stranded Total RNA Sample Preparation Kit protocol on Biomek i7 Dual Hybrid (Multichannel 96, Span-8) Genomics Workstation.
Biomek i-Series Automation of the Beckman Coulter Agencourt DNAdvance Genomic DNA isolation Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this technical note, we demonstrate automated performance of DNAdvance Tissue Kit on Biomeck i series.
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features Biomek i-Series automated Illumina TruSeq DNA PCR-Free with the spotlight on the Biomek i5 Span-8 Genomic Workstation
Biomek i-Series Automated New England Biolabs NEBNext® Ultra IITM DNA Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this technical note, we demonstrate automated performance of the Kit on the Biomek i7 Dual Hybrid (Multichannel 96, Span-8) Genomics Workstation.
Biomek i-Series Automation of the Apostle MiniMax™ High Efficiency cfDNA Isolation Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this technical note, we demonstrate the automated performance of the Apostle MiniMax™ High Efficiency cfDNA Isolation Kit on the Biomek i7 Hybrid Genomics Workstation.
Detection Limit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For Detection Limit (DL), often referred to as Limit of Detection (LOD), both ICH and JP 16 offer clear direction.
Control Standards and Method Recommendations for the LS 13 320 XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Echo System-Enhanced SMART-Seq v2 for RNA Sequencing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here, we demonstrate miniaturization of this process on human brain total RNA utilizing the Echo 525 Liquid Handler. We show that this enhanced process can effectively reduce reagent cost for the workflow, and see that genes are not differentially expressed across miniaturization and input variables as shown in Illumina RNAExpress analysis in BaseSpace.
Using k-Factor to Compare Rotor Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The most important contributors to rotor efficiency are the maximum speed, maximum radius (rmax), and minimum radius (rmin)—all of which contribute to the maximum g-force generated by the rotor.
Inorganic Carbon Removal {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	To quantify TOC, all analyzers must incorporate a strategy to account for the TIC. However, not all of these strategies are equally effective or reliable.
Scalable Plasmid Purification using CosMCPrep {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the flexibility of CosMCPrep, a scalable plasmid purification kit
Optimizing Workflow Efficiency of Cleanroom Routine Environmental Monitoring {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper examines a thoughtfully designed and well-executed environmental monitoring program that tests the controls put in place to maintain the cleanroom to the required Good Manufacturing Practices standards (e.g., CGMP, EU-GMP, and PIC/S).
What to do now that ACFTD is discontinued {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note will examine the steps that optical Particle Counter users need to take to transition from an ACFTD calibration to an ISO-MTD calibration, the modern equivalent.
Vi-CELL BLU Regulatory Compliance - 21 CFR Part 11 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The following information describes the relevant portions of the 21 CFR Part 11 regulations and their implementation using the Vi-CELL BLU control software.
Modern Trends in Non‐Viable Particle Monitoring during Aseptic Processing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper describes changes and improvements to non‐viable particle monitoring (NVP), sometimes referred to as total particulate monitoring, which is a regulatory requirement during aseptic processing. Aseptic processing is becoming more automated and increasingly important to future products of the biopharmaceutical industry. In the same manner, NVP monitoring is also becoming more automated and increasingly important for contamination risk management during aseptic processing.
Vaporized Hydrogen Peroxide Decontamination of Vi–CELL BLU Instrument {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the method and results for testing the Vi-CELL BLU for VHP resistance.
Switching from Oil Testing to Water and back using the HIAC 8011+ and HIAC PODS+ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Specifying Non-Viable Particle Monitoring for Aseptic Processing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper details key considerations when implementing a continuous non-viable particle monitoring (NVP) system.
Method for Determining Cell Type Parameter Adjustment to Match Legacy Vi CELL XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details how you can use the plate loader feature on the Vi-CELL BLU to match cell types to give cell counts and viability levels equivalent to those obtained on existing Vi-CELL XR instruments.
g-Max: Added Capabilities to Beckman Coulter's versatile Ultracentrifuge Line {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The g-Max System adds several valuable capabilities to your Beckman Coulter ultracentrifuge rotors:
Biomek i-Series Automated Illumina TruSeq® Stranded mRNA Sample Preparation Kit Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this technical note, we present the automation of the Stranded mRNA Sample Preparation Kit protocol on Biomek i7 Dual Hybrid (Multichannel 96, Span-8) Genomics Workstation.
Importance of TOC measurement in WFI in light of European Pharmacopoeia change {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper discusses the increased focus on Total Organic Carbon (TOC) and conductivity measurement, especially for companies seeking to use RO water treatment plants to create WFI, and provides best-practice advice for calibration.
Biomek i-Series Automated Illumina Nextera DNA Flex Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this flyer, we demonstrate the automated performance of Illumina Nextera DNA Flex Library Prep Kit on the Biomek i7 Hybrid Genomics Workstation.
Optimizing the Multisizer 4e Coutler Counter for use with Small Apertures {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The purpose of this application note is to provide some guidelines to facilitate successful testing with the Multisizer 4e when employing 30 µm and smaller.
A workflow for medium-throughput isolation of cfDNA from plasma samples using Apostle MiniMax™ on the KingFisher™ Technology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of Apostle MiniMax™ High Efficiency cfDNA Isolation Kit, in conjunction with the KingFisher Duo Prime automated protein purification system. This potential solution that mitigates some of the challenges of processing large volume samples. Automating the chemistry can also reduce the risk of human error, reduce hands-on time and total time; therefore giving the user the ability to run more samples in a day.
Critical Particle Size Distribution for Cement using Laser Diffraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Biomek i-Series Automated KAPA HyperPrep and HyperPlus Workflows {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The KAPA HyperPrep and HyperPlus Kits allow users to prepare DNA libraries compatible with Illumina sequencing platforms from a variety of sample types (including high-quality genomic DNA, FFPE derived DNA, etc.) and a wide range of input amounts (1 ng to 1 µg).
Particle Size Analysis Simple, Effective and Precise {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
HIAC PODS+ Online Mode & Filter Cart Mode {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The HIAC PODS+ has three different sampling modes, Bottle Mode, Online Mode and Filter Cart Mode. Knowing the differences between Online Mode and Filter Cart Mode will allow operators to get the most out of their PODS+.
Pharma Manufacturing Paperless Monitoring {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Grading of pigment ink and measurement of particle diameter using ultracentrifugation / dynamic light scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
A Highly Consistent Lowry Method on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Lowry method has been widely used for protein quantification for many years. The quantification is based on peptide bonds of proteins reacting with copper under alkaline conditions followed by copper-catalyzed oxidation of aromatic amino acids, producing blue color. The intensity of the blue color is measured at 750 nm and it is proportional to the protein quantity. The repetitive pipetting and incubation steps in the Lowry method makes it extremely hands-on and time consuming, when done manually.
Beer, Evaluation of Final Product and Filtration Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The concentration and size distribution of particles in beer may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method
Overload Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Experienced operators can develop reasonable expectations about what the TOC values will be and the measurement values will tend to fall within an expected range most of the time.
Minimal Sample to Sample Carry Over with the HIAC 8011+ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sample to sample carry over is a significant contributor to inaccurate and unreliable particle count measurements in industrial fluids which can severely impact manufacturing operations, maintenance, and quality control costs. The HIAC 8011+ has been specifically designed to dramatically minimize this contribution of contamination.
Grading of nanocellulose using a centrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
HIAC PODS+ versus Parker ACM-20 Performance comparison {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The HIAC PODS+ has new capabilities with the industry’s fastest sample to answer. Fast sample means higher throughput translating into higher efficiency and consequently lower costs. Let’s take a peek at the performance specs, chapter and verse, and let the feature and function differentiation speak for itself.
Consistent Cell Maintenance and Plating through Automation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell culture is a valuable yet time-consuming aspect of basic biological research and drug discovery. Whether short- or long-term assays, the maintenance of cells is a frequently recurring process and the workload and possibility for contamination increases as the number of cell lines being used by a laboratory increases. In addition, different scientists frequently use different techniques when maintaining cell lines and these alterations can manifest in subsequent assay variability when using these cells.
A Highly Consistent BCA Assay on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The BCA (bicinchoninic acid) assay is a popular method used in quantification of proteins. The quantification is based on the purple color developed due to the chelation of copper ions by reduction bicinchoninic acid. The resulting complex absorbs at 562 nm and the optical density is proportional to the protein concentration in the sample. As BCA is not an end point method, the color continue to develop, even after the incubation.
Preparation and purification of carbon nanotubes using an ultracentrifuge and automatic dispensing apparatus, and analysis using an analytical centrifuge system {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Separation and purification of metal nanorods using density gradient centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Biomek i–Series Automated Illumina® TruSight Tumor 170 32 Sample Method {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We demonstrate the automated performance of Illumina TruSight Tumor 170 32 Sample Method on the Biomek i5 Span–8 Genomics Workstation.
Particle diameter measurement of a nanoparticle composite - Using density gradient ultracentrifugation and dynamic light scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Full Automation of the SISCAPA® Workflow using a Biomek NXP Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features the Biomek NX<sup>P</sup> Automated Workstation and the full automation of the SISCAPA® workflow
Optimizing the HIAC 8011+ Particle Counter for Analyzing Viscous Fluids {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	There are 3 primary elements that directly impact the HIAC 8011+’s ability to analyze viscous fluids; sensor aperture size, flow rate, and the HIAC 8011+ instruments pressure settings. Configuration design options and internal operator selectable features will optimize the instrument to allow measurement capability across a wide range of fluids and viscosities.
ICH Q2 – The Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Highly Reproducible Automated Proteomics Sample Preparation on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Protein digestion and analysis by mass spectrometry is an essential process in numerous proteomic approaches such as biomarker discovery. However, the sample preparation can be tedious and error prone. Automation can relieve the burden of sample preparation while also reducing the variability between users and across experiments.
Matching Cell Counts between Vi–CELL XR and Vi–CELL BLU {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper demonstrates a series of different cell types run on the Vi-CELL XR and duplicate samples run on the Vi-CELL BLU. The cells were then reanalyzed adjusting the cell type parameters until a new cell type was generated that gave cell concentrations within +/–5% and viable cell density levels within +/–2.5% of the Vi-CELL XR.
Illumina TruSight Oncology 500 Automated on the Biomek NXᴾ Span-8 Genomics Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this flyer, we demonstrate the automated performance of Illumina TruSight Oncology 500 assay on the Biomek NX<sup>P</sup> Span-8 Genomics Workstation.
Fully-Automated Cellular Analysis by Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Specification Comparison of Vi–CELL XR and Vi–CELL BLU {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Vi–CELL BLU leverages the key performance features of the Vi–CELL XR but incorporates many design improvements that our customers have requested over the years. This paper highlights the differences in specifications between the two instruments.
Considerations of Cell Counting Analysis when using Different Types of Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper reviews several different cell types and cell preparation approaches using standard cells, such as, CHO cells, EL4 cells, SF9 cells, and HELA cell cultures.
Root Cause Investigations for Pharmaceutical Water Systems {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this paper we discuss some typical sources of contamination in water systems and how root-cause investigations can be supported using on-line Total Organic Carbon (TOC) analysers.
Evaluation of Instrument to Instrument Performance of the Vi-CELL BLU Cell Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details one of the key strengths of the Vi–CELL BLU, the ability to fine tune the performance to minimize variability between different instruments. This is particularly valuable in facilities that utilize multiple instruments but can also be important when instruments are occasionally shared between departments.
Cell Line Development – Hit Picking {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note we discuss how hit picking was automated as part of a cell line development workflow
Quality Control Electronic Records for 21 CFR part 11 Compliance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper describes how Quality Control instruments can be optimised for pharmaceutical use, helping to improve the integrity of the data in the final electronic record.
A Standardized, Automated Approach For Exosome Isolation And Characterization Using Beckman Coulter Instrumentation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Exosomes are small microvesicles, derived from the late endosome, most often described in the literature to be less than 120 nm, released by all cell types, and proven to be involved in cancer metastasis.
Biomek i-Series Automated SurePlex PCR and VeriSeq PGS Library Prep for Illumina® {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this flyer, we demonstrate the automated performance of Illumina® SurePlex PCR and VeriSeq Library Prep Kit on the Biomek i5 Span-8 Genomics Workstation.
Determination of Size and Concentration of Particles in Oils {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The concentration and size distribution of particles in an oil sample may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method
Issues with Testing Jet Fuels for Contamination {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This page explains how the HIAC PODS+ Particle Counter can give you an accurate measurement of particle contamination and has the capability of detecting moisture as well. Do you want to know if your fuels has both water and contamination? Then see how the new HIAC PODS+ provides the information you need.
Isolation of cell-free DNA (cfDNA) from plasma using Apostle MiniMax™ High Efficiency cfDNA Isolation kit— comparison of fully automated, semi-automated and manual workflow processing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note compares workflows and yield for the extraction of cfDNA using Apostle MiniMax™ High Efficiency cfDNA Isolation Kit, following the manual protocol, automating the extraction using the Biomek i7 Hybrid Workstation, and semi-automating the extraction using the KingFisher™ Duo Prime Sample Purification System.
Temperature dependence of hydrodynamic radius of an intrinsically disordered protein measured in the Optima AUC analytical ultracentrifuge. {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Analytical ultracentrifugation experiments over a wide range of temperatures were conducted to measure the hydrodynamic radius of an intrinsically disordered protein. Taking advantage of the highly stable temperature control offered through the latest shroud modification available in the new Optima AUC, replicate experiments demonstrated highly reproducible hydrodynamic parameters that suggest slight changes in the hydrodynamic radius of an intrinsically disordered protein, and impressive performance in both temperature control and data quality from the Optima AUC.
Calibrating the QbD1200 TOC Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The calibration of a TOC Analyzer is a crucial part of ensuring a quality measurement.
Particle Counting in Mining Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	How Particle Counters can Extend Machine Uptime, Oil Lifetime and Give More Reliability
Enumeration And Size Distribution Of Yeast Cells In The Brewing Industry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Breweries, such as those operated by Anheuser Busch, Inc., utilize the Coulter Principle for their yeast cell enumeration and sizing
Cell Line Development – Limiting Dilution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Limiting dilution is a traditional approach to achieve monoclonality. It entails diluting cells to the level that there is one cell on average per unit of volume plated in the wells. However, as predicted by the Poisson distribution, roughly one third of the plated wells will have zero cells, one third of wells will have a single cell, and one third will have multiple cells per well (Coller and Coller 1986).
Avoid the Pitfalls When Automating Cell Viability Counting for Biopharmaceutical Quality Control {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this paper we will show one example of how identifying viable cells by minimum diameter alone can introduce variability of circa 33% in the reported cell concentration. This paper describes how to avoid these common pitfalls when a user is adopting a new automated method of cell counting.
Cell Line Development – Hit Picking {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Flexible ELISA automation with the Biomek i5 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Enzyme-linked immunosorbent assays (ELISAs) are a common analytical tool for detecting the presence of a protein or other molecules of interest. While the workflow is broadly conserved, numerous reagent addition and wash steps make this a labor-intensive endeavor. Automation of these steps not only relieves this labor burden but can lead to improvements in result consistency.
A method of grading nanoparticles using ultracentrifugation in order to determine the accurate particle diameter {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
A Highly Consistent Bradford Assay on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Bradford protein assay is a simple, fast and sensitive method that is used to measure the concentration of total protein in a sample. The principle of this assay is the binding of protein molecules to Coomassie dye under acidic conditions, resulting in a color change from brown to blue. Due to its robustness, this assay has become the preferred method for protein quantification. Although simple, the repetitive pipetting steps in the Bradford assay makes it cumbersome to carryout manually, especially when the throughput is high.
Cell Line Development – Selection and Enrichment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell line development is a highly involved and inefficient process. The quest to find cells that stably express and secrete antibodies or other protein products from a transfected pool of cells requires the screening of thousands of wells/cells.
Cell Line Development – Data Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell line development is one of many workflows that has significant data challenges. Like any screening process, the sample throughput provides a challenge from the sheer amount of data. This becomes even more challenging when multiple analytical instruments are used to generate different types of data, often in different formats.
Efficient Factorial Optimization of Transfection Conditions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nucleic acid transfection is a procedure used in nearly any cellular laboratory and the abundance of commercially available reagents has made this a seemingly simple endeavor. However, whether one is transfecting plasmid DNA or inhibitory RNAs, different cell lines can have significant differences in the ease of transfection or cellular survival. Determining the optimal plating conditions for high transfection efficiency and low cytotoxicity can be highly involved.
Metal colloid purification and concentration using ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Minimizing process variability in the manufacturing of bottled drinking water {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This resource discusses performing particle count measurements at many critical points throughout the process of producing Bottled Drinking Water. Depending upon the supplier and the nature of their final product, each will have a set of unique metrics or critical process points that can be monitored for particle counts and analyzed to assess repeatability and reproducibility which in turn can demonstrate control of the process and lead to a consistent quality of their finished products.
Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note presents how our media analyzer Vi-CELL MetaFLEX was tested and the potential benefits for accelerating metabolic rewiring research.
ICH Q2 – the Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This page discusses some of the challenges when using TOC analysers to demonstrate pharmacopoeial TOC level compliance for modern water systems in the light of the ICH Q2 document2 from the International Conference on Harmonisation.
Exosome-Depleted FBS Using Beckman Coulter Centrifugation: The cost-effective, Consistent choice {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Exosomes are small microvesicles, derived from the late endosome, most often described in the literature to be less than 120 nm, released by all cell types, and proven to be involved in cancer metastasis.
European Pharmacopoeia EP 2.2.44 and Total Organic Carbon {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper discusses the background behind the European Pharmacopoeia’s focus on complete oxidation of TOC in the light of the increased importance of on-line Total Organic Carbon (TOC) and conductivity measurement.
Automating the Cell Line Development Workflow {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features the Biomek i-Series Automated Workstation - a complete walk-away solution for your cell line development workflow
Changes to GMP Force Cleanroom Re-Classifications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper explains the rules of ISO 14644-1:2015, which has substantial revisions impacting both the way the cleanrooms are classified and the performance requirements of the air particle counting instruments used to carry out the classification.
Detecting Moisture in Hydraulic Fluid, Oil and Fuels	Monitoring the amount of moisture in petroleum based fluids before 100% saturation occurs will reduce degradation and will increase the life and performance of equipment components. Advancements in technology allowed Beckman Coulter to add a moisture sensor to our HIAC PODS+ Portable Liquid Particle Counters. This application note details the moisture detection application in hydraulic fluid, oil and fuels.
Biomek Automated NGS Solutions Accelerate Genomic Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features the Biomek i7 automated genomic workstation and focuses on automated NGS solutions
Cellular Analysis using the Coulter Principle {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Coulter Principle is based on the detection and measurement of changes in electrical resistance produced by a particle or cell suspended in a conductive liquid (diluent) traversing through a small aperture.
Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Technical Information Bulletin by Matthew Peloquin, Sanford-Burnham Medical Research Institute, Orlando, Florida, USA.
Fully Automated Peptide Desalting for Liquid Chromatography–Tandem Mass Spectrometry Analysis Using Beckman Coulter Biomek i7 Hybrid Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes a completely hands-free automated procedure of the peptide desalting process needed for the peptide clean-up sample preparation steps of LCMS/ MS.
Automated Research Flow Cytometry Workflow Using DURA Innovations Dry Reagent Technology with the Biomek i7 Automated Workstation and CytoFLEX LX Flow Cytometer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Learn about DURA Innovations dry reagent technology and see how dry reagents can be used in an automated flow cytometry workflow.
Use Machine Learning Algorithms to Explore the Potential of Your High Dimensional Flow Cytometry Data Example of a 20–color Panel on CytoFLEX LX	Explore the potential of high dimensional flow cytometry data with an Example of a 20–color Panel on CytoFLEX LX. Understand how to perform machine learning algorithms like viSNE and FlowSOM to identify phenotypes of populations/subsets present in the 20–color CytoFLEX LX flow cytometry data. Build a computational flow cytometry data analysis pipeline with Cytobank. Learn how to assess the quality of viSNE maps and FlowSOM clustering results. Recognize how pre–processing steps can affect the result quality of machine learning algorithms.
Biomek i7 Hybrid Automated KAPA mRNA HyperPrep Workflow {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this flyer, the automated performance of the KAPA mRNA HyperPrep kit on the Biomek i7 Hybrid Genomics Workstation is demonstrated through successful library preparation and sequencing.
Miniaturization of an Epigenetic AlphaLISA Assay with the Echo Liquid Handler and the BMG LABTECH PHERAstar FS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Epigenetics continues to emerge as an important target class for drug discovery and cancer research. As programs scale to evaluate many new targets related to epigenetic expression, new tools and techniques are required to enable efficient and reproducible high-throughput epigenetic screening. The combination of robust and precise instrumentation from Beckman Coulter Life Sciences and BMG LABTECH enable the miniaturization and automation of PerkinElmer AlphaLISA epigenetic assay kits without compromising performance and data quality.
Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 555 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Results presented here demonstrate that miniaturized and simplified timedependent CYP inhibition assays can be efficiently conducted with the Echo Liquid Handler.
High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Results presented here demonstrate that miniaturized and simplified time-dependent CYP inhibition assays can be efficiently conducted with the Echo Liquid Handler.
Miniaturized and High-Throughput Metabolic Stability Assay Enabled by the Echo Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we discuss how the Echo Liquid Handler provides a platform for conducting a miniaturized high-throughput metabolic stability assay. The Echo Liquid Handler precisely and accurately transfers nanoliter volumes with acoustic energy in a completely contact-free manner. Thus high quality data can be obtained with a small fraction of the volume required by tip-based liquid handling methods.
cfDNA Extraction Efficiency Affects NGS Data	An increasing number of researchers indicate that the use of cfDNA and circulating tumor DNA (ctDNA) for cancer detection, prognosis and monitoring positively impact the clinical management and outcomes of patients.
Miniaturization and Rapid Processing of TXTL Reactions Using Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application Note: Using the Echo 525 Liquid Handler in combination with the myTXTL Master Mix provides a comprehensive solution for gene expression projects that require rapid high-throughput processing, flexibility, and reproducibility in a low cost assay
Optimized NGS Library Preparation with Acoustic Liquid Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Radiant Genomics and Beckman Coulter Life Sciences have developed a low-volume, multiplexed next-generation sequencing (NGS) library preparation method using the Echo 525 Liquid Handler. In addition to cost savings, we demonstrate an increase in sample-processing throughput with the Echo Liquid Handler due to efficient multiplexing, which would otherwise remain impractical using standard liquid handling automation. In addition, the barcoding and combinatorial pooling strategy outlined here can be applied to workflows beyond NGS.
Miniaturized Enzo Life Sciences HDAC1 Fluor de Lys Assays Using an Echo Liquid Handler Integrated in an Access Laboratory Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The screening effort described in this application note utilized the 2.5 nL drop size capability of the Echo 555 Liquid Handler to dispense compounds and the larger volume 25 nL drop size capability of an integrated Echo 525 to dispense aqueous epigenetic reagents in an automated screening assay using the Access Laboratory Workstation.
Assay Assembly for Miniaturized Quantitative PCR in a 384-well Format Using the Echo Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note highlights the capabilities of the Echo 555 Liquid Handler to dispense into 384-well qPCR plates with total reaction volumes as low as 250 nanoliters. Standard deviations of less than 0.25 and CVs of less than 2% are seen routinely across plates using the low volume dispensing of the Echo Liquid Handler.
Miniaturized EPIgeneous HTRF Assays Using the Echo Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cisbio’s HTRF assay technology has a wide range of applications in cell-based and biochemical assays. These assays are readily adaptable to high-throughput screening in 384-well and 1536-well formats. The Echo liquid handler miniaturized HTRF assays to 2 μL assay volumes in 1536-well microplates. Reagents and compounds were transferred at 2.5 nL increments, which enabled flexibility in assay setup and reduced reagent costs. The Set7/9 methyltransferase was used as a model target to verify performance of a miniaturized epigenetic assay.
Miniaturized Genotyping Reactions Using the Echo Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Echo Liquid Handler enables low volume genotyping reactions in a 384-well format with as little as 500 nL total reaction volume. Precise and accurate drop placement eliminates cross-contamination, while offering the flexibility to transfer from any well of a source plate to any well of a destination plate.
Nanoliter Scale DNA Assembly Utilizing the NEBuilder HiFi Cloning Kit with the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The goals of these experiments were to utilize the Echo 525 Liquid Handler in assembling both a two-piece and five-piece assembly in miniaturized reaction volumes.
Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter Volumes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The goal of this experiment was to utilize the Echo 525 Liquid Handler in assembling five, five-piece assemblies by substituting modular DNA pieces at the nanoliter scale.
Effective Miniaturization of Illumina Nextera XT Library Prep for Multiplexed Whole Genome Sequencing and Microbiome Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we detail a method to miniaturize reaction volumes utilized in the Illumina Nextera XT DNA library kit, thereby reducing the overall cost per reaction while maintaining equivalent data to the standard protocol.
dsDNA Quantification with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The precision and accuracy of the miniaturized reaction volumes for the two kits in an Echo 525 Liquid Handler-enabled workflow was verified by quantifying a library of dsDNA samples in a 384-well polypropylene microplate and cross checking the quantification data against the Qubit dsDNA (HS) Assay Kit.
Automating Biopharma Quality Control to Reduce Costs and Improve Data Integrity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper takes a look at four common quality control procedures and how automation can help improve data integrity by reducing opportunities for human error, whilst also saving time and reducing operating costs.
Media optimization in the RoboLector® Pro for enhanced protein production using C. glutamicum {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In the research project described in this application note, we are presenting a media optimization of the minimal medium for <em>Corynebacterium glutamicum</em> (CGXII medium) using the RoboLector® and BioLector® Pro. The media optimization in the DoE is focused on increasing the cutinase activity in <em>C. glutamicum</em>. The organism is cultivated in batch and fed-batch processes using the BioLector® Pro in order to investigate the influence of the media components and feeding rates on the growthrelated secretory protein production of cutinase. In this context, we further demonstrate a successfully performed microbial cultivation within the high cell density range in micro liter scale.
Screening of yeast-based nutrients for E. coli-based recombinant protein production using the RoboLector® {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As shown in this App-Note media screening can be conducted with minimal effort. With the integrated liquid handler even more opportunities are given. Feeding, pH regulation and - as shown in this application note - induction profiling can be implemented in small scale, giving the user the advantage of saving time and costs.
Новый режим торможения в центрифуге Avanti J-15 экономит время и повышает эффективность работы {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Эти рекомендации по применению демонстрируют, как новый режим торможения Avanti J-15 экономит ваше время.
Решения для производства и разработки вакцин {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Высокопроизводительные и ультрацентрифуги для разработки и производства вакцин
Автоматизированный анализ посева и роста клеток {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Демонстрация автоматизации серийного разведения, посева и роста клеток, а также подготовки к анализу методом XTT с помощью Biomek i5.
Ускорение ваших геномных исследований с автоматизацией пробоподготовки на Biomek {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Комплексное решение для очистки белков с центрифугой Avanti JXN {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Центрифугирование – это важный и, как правило, первый этап протокола очистки белка. Центрифуги серии Avanti JXN позволяют упростить работу на любой стадии выделения.
Ускорение исследований с автоматизированными станциями Biomek для геномики {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Методическое пособие по автоматизации контроля качества и нормализации образцов на Biomek i-Series
Революция в аналитическом ультрацентрифугировании: новая центрифуга Optima AUC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Аналитическая ультрацентрифуга Optima AUC – одна из последних разработок компании Beckman Coulter. Благодая своим преимуществам она обеспечивает значительные преимущества по сравлению с предшествующей системой ProteomeLab
Соответствие требованиям GMP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Центрифуги серий Optima XPN и Avanti JXN помогают соответствовать требованиям GMP и избавляет от лишних хлопот.
Новая центрифуга Avanti J-15 повышает сохранность и максимизирует выход образцов {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Beckman Coulter Life Sciences демонстрирует как центрифуга Avanti J-15 повышает сохранность и максимизирует выход образцов
Комплексное решение для очистки белков с центрифугой Avanti JXN {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Центрифугирование – это важный и, как правило, первый этап протокола очистки белка. Центрифуги серии Avanti JXN позволяют упростить работу на любой стадии выделения.
Принцип проточного центрифугирования {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Проточное центрифугирование экономит время работы оператора, исключая необходимость постоянно заполнять и опорожнять многочисленные центрифужные бутыли, а также запускать и останавливать процесс.
Алгоритмы машинного обучения обеспечивают глубокое понимание состава клеточных субпопуляций при использовании 20-цветного иммунофенотипирования {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Utilization of the MicroClime Environmental Lid to Reduce Edge Effects in a Cell-based Proliferation Assay {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this study, we demonstrate the benefit of utilizing the MicroClime Environmental Lid to reduce edge effects in a functional cell-based assay by measuring the mitigation of evaporation of fluid in reaction wells.
Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube-based DURAClone dry reagent {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Using the 10 color DuraClone IM T Cell Subsets kit plus 3 additional liquid markers, a 13 color tube has been designed for the phenotypic analysis of major T cell sub-populations in the human peripheral blood.
Whole Genome Sequencing of Microbial Communities for Scaling Microbiome and Metagenomic Studies Using the Echo 525 Liquid Handler and CosmosID {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this study, we demonstrate that the Echo 525 Liquid Handler can be used to miniaturize WGS of microbial communities. Raw data can be processed, analyzed, and ready for interpretation within the hour, by utilizing CosmosID’s best-in-class microbiome bioinformatics platform.
Miniaturized 16S rRNA Amplicon Sequencing with the Echo 525 Liquid Handler for Metagenomic and Microbiome Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here, we perform a standard Illumina 16S rRNA amplicon sequencing library preparation at miniaturized scale using the Echo 525 Liquid Handler, effectively reducing reaction volumes and input sample while maintaining sufficient read depth to accurately capture the community. The result is a significantly cost-reduced workflow per sample, saving reagent costs, operational time costs, and valuable sample DNA.
Miniaturized Multi-Piece DNA Assembly Using the Echo 525 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In the studies described in this Application Note, the NEB NEBuilder HiFi DNA Assembly Cloning Kit was combined with the Echo 525 Liquid Handler to assemble a construct over five modular pieces.
Nanoliter Scale High-Throughput Protein Crystallography Screening with the Echo Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Precise transfer of reagents ensures repeatable crystallization trials. With Dynamic Fluid Analysis only a single fluid class setting is needed to transfer all crystallography sparse matrix reagents—eliminating the need for multiple calibrations or repeated optimization of fluid class settings. In addition to transferring sparse matrix reagents, the Echo liquid handler can also use Dynamic Fluid Analysis to transfer high concentrations of stock reagents on-the-fly, which allows operators to create optimization grid screens without individually making all variations.
Miniaturized Sequencing Workflows for Microbiome and Metagenomic Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Echo® 525 Liquid Handler can be employed to miniaturize workflows, thus decreasing reagent cost and time spent per sample. Our results show that miniaturizing sequencing workflows is possible and yields high quality data. Here Echo 525 Liquid Handler enables the throughput needed for modern microbiome studies by reducing the time and cost per sample and processing them with speed and accuracy.
Miniaturized Gene Expression in as Little as 250 nL {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Access workstation combines the Echo liquid handler, the Roche RealTime ready Cell Lysis Kit and the Roche LightCycler 480 system into a single automated system for gene expression assays with reaction volumes as low as 250 nL. With flexible, wizard–driven software and high quality acoustic liquid transfer, the Access workstation is an ideal platform for both assay development and screening with zero cross contamination.
Miniaturized Enzymatic Assays with Glycerol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application highlight discusses the capability of the Echo liquid handlers to transfer low volumes of enzyme stored in 50% (v/v) glycerol into 384-well plates. This allows both the simplification of protocols by removal of an intermediate dilution step, and improved performance in a miniaturized format.
High throughput cultivation of the cellulolytic fungus Trichoderma reesei in the BioLector® {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note we show, that the BioLector® system is suitable for high throughput fermentation of <em>T. reesei</em> while maintaining essentially the same morphology, cellulase production and growth kinetics as in conventional shake flask fermentations. Furthermore, we demonstrate different application examples that take advantage of the optical online measurement capabilities of the BioLector®.
Determination of cell death in the BioLector® II/ Pro {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note m2p-labs presents the online propidium iodide (PI) measurement during cultivation processes using the BioLector® Pro. This method promises a rapid signal response, applicable for example in high throughput cytotoxicity screenings in 48 well format.
Low-pH profiling in µL-scale to optimize protein production in H. polymorpha using the BioLector® Pro {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note a pH profiling for a maximized green fluorescent protein (GFP) yield in H. polymorpha has been conducted using the microbioreactor BioLector® Pro. Within this highthroughput fermentation study, we investigated strains of <em>H. polymorpha</em> containing the FMD and the MOX promoter, to determine the preferred pH range for efficient protein production. Up to now, the investigated low pH range between pH 4 to 6 has been very challenging to measure and control in small scale cultivation systems. Here, optical pH optodes are the measurement system of choice.
Preparation of Mouse Plasma Microsamples for LC-MS/MS Analysis Using the Echo Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In response to programs like the EU 3Rs initiative to reduce the use of animals in research researchers have turned to microsampling as an alternative to composite studies. With advances in bioanalytical techniques drug levels can be determined from samples of 20 μL or less. This small sample volume or microsample enables reduces the dependency on satellite animals to complete a study. Additionally, microsampling is faster and less stressful than traditional composite studies.
Beckman’s LS 13 320 XR Vs. Malvern Mastersizer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}

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