Quick Reference Guide: Genedrive® 96 SARS-CoV-2 Kit

Step 1

Step 1

Place 96-well plate in a cool block for the duration of reaction setup.
 
Step 2

Step 2

Carefully peel back the foil seal. It is recommended to do this in sections to minimise open wells during pipetting. Check there is a reagent bead in each well.
Step 3

Step 3

Pipette 20µL of extracted specimen or control into the side of each well. Add 20µL of nuclease free water to any wells that do not contain a specimen. Ensure all beads have been rehydrated.
Step 4

Step 4

Carefully peel optical film seal from its backing and seal the plate. Use of a microtitre sealing brayer or similar is recommended.
Step 5

Step 5

Carefully drop cool block 3 times from 1 cm height. Vortex the cool block/plate for 3 x 15 seconds at full power (total 45 seconds).
Step 6

Step 6

Carefully drop cool block 3 times from 1 cm height. Incubate on cool block for 5 minutes.
Step 7

Step 7

Vortex the cool block/plate for 3 x 15 seconds at full power (total 45 seconds).
Step 8

Step 8

Centrifuge the plate 2 minutes at 2000 RPM.
Step 9

Step 9

Load the plate on the Real-Time PCR instrument and start the program immediately. Ensure all the run parameters are correct before starting.
Disclaimers:
CE-IVD: Products intended for in vitro diagnostic use and conforming to European Directive (98/79/EC).
US EUA: Validation of this test has not been reviewed by FDA. Review under the EUA program is pending. For In Vitro Diagnostic (IVD) Use. Rx Only.

 

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