Выделение ДНК из FFPE ткани

Agencourt FormaPure DNA

Вы можете использовать наш запатентованный химический анализ SPRI на основе парамагнитных частиц для эффективной и последовательной экстракции ДНК из фиксированных формалином, залитых парафином тканей.

ДНК, экстрагированная при помощи FormaPure DNA, подходит для дальнейших применений с самыми жесткими ограничениями, включая NGS
Сохранение выявляемости низкочастотных вариантов без экспериментальной погрешности измерения с учетом экстракции
Совместимость с ручной и автоматизированной обработкой (включая депарафинизацию и лизис проб)
Производительность продемонстрирована при использовании самых различных типов проб и тканей

FormaPure XL DNA Visual Workflow

Genomics FormaPure XL DNA Workflow

FormaPure XL DNA Features

Обзор
Набор FormaPure DNA позволяет выделять ДНК из различных фиксированных формалином, залитых парафином (formalin-fixed, paraffin-embedded, FFPE) проб и типов ткани.

Упрощенный рабочий процесс
FormaPure DNA предлагает удобный ручной рабочий процесс с минимальными затратами времени без применения летучих химических веществ.

Product Specifications: FormaPure XL DNA

Application Uses	DNA Extraction, DNA Isolation, DNA sequencing, Nucleic acid sample prep
Format	Жидкий
Starting Sample Material	FFPE (formalin fixed paraffin embedded) tissue.

Automated Available	Yes
Item Specifications Referenced	C35996

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Название	Описание
Agencourt Genfind v2 System Genomic DNA Isolation Kit {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	High recovery of DNA for downstream applications.
DNA extraction from up to 2 mL blood using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Here we present a highquality, research-ready DNA extraction method from large volume whole blood by using GenFind V3.
DNA extraction from Zeesan@ Saliva DNA sample collection kit using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Saliva contains buccal epithelial cells and white blood cells which provide rich genetic data. Due to the easy access and non-invasive character, saliva is an alternative to blood collection. Here we present a high-quality, DNA extraction method from saliva using GenFind V3.
Total Nucleic Acid Isolation from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues using FormaPure XL Total on a KingFisher™ Duo Prime {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol demonstrates the FormaPure XL Total performance on the KingFisher Duo Prime purification system. Automating chemistry can reduce the risk of human error, hands-on time (HOT) and total turn-around time (TAT).
Whole Blood DNA Extraction from PAXgene Blood DNA Tube using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Here we show that the GenFind V3 protocol can process whole blood directly from PAXgene™ Blood DNA Tubes with no centrifugation step necessary
DNA extraction FTA cards or filter paper blood spots using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This method provides instruction for additional lysis buffer volumes and optimized lysis conditions for extraction of DNA
DNA extraction from Mouthwash samples using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Mouthwash samples are taken in large volumes (usually 10 - 30 mL), and cells need to be spun down from the total volume of mouthwash before extraction can begin. This protocol incorporates those steps and optimizes the lysis for mouth cell samples
Extraction of DNA from Mucoid Bacteria using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol reduces viscosity due to the presence of exopolysaccharides and decreases the amount of polysaccharide in the final eluate.
Formalin-fixed and non-paraffin-embedded tissue DNA extraction {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Here we demonstrated a DNA extraction protocol from formalin-fixed and nonparaffin-embedded brain tissue using FormaPure DNA XL kit. In this protocol, protein-DNA cross-links are reversed using heat
DNA extraction from fresh/frozen tissue using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This provides a solution that allows a user to use one kit to extract DNA from multiple sample types with small modifications in the workflow.
Cell-Free DNA (cfDNA) Isolation from Plasma using Apostle MiniMax™ High Efficiency cfDNA Isolation Kit on a King Fisher™ Duo Prime {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol demonstrates the Apostle MiniMax High Efficiency cfDNA Isolation Kit performance on the KingFisher Duo Prime purification system.
Simultaneous DNA & RNA Extraction from Cultured Cells without Splitting Lysate {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	We developed a protocol using Beckman Coulter reagents to isolate both DNA and RNA from the same cultured cell sample. The method utilizes a proprietary buffer to selectively bind RNA and DNA
DNA extraction from cell culture using GenFind V3 {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	This protocol provides a method for extracting DNA from cultured cells (≤ 2 million cells). Data shown shows the efficiency of this procedure with 1.4 million Jurkart cells, an immortalized line of human T lymphocyte cells
Automated library preparation for the MCI Advantage Cancer Panel at Miami Cancer Institute utilizing the Beckman Coulter Biomek i5 Span-8 NGS Workstation {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Here we describe the automation of the MCI Advantage Cancer Panel at Miami Cancer Institute, a 170 gene panel assay based upon the Illumina TruSight® Tumor 170 Panel, on the Beckman Coulter Biomek i5 Span-8 NGS Workstation
Automating Genomic DNA Extraction from Whole Blood and Serum with GenFind V3 on the Biomek i7 Hybrid Genomic Workstation {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Here, we demonstrate a walk-away automated solution for GenFind V3 kit to purify up to 400 µL of whole blood using the Beckman Coulter Biomek i7 Hybrid Genomics Workstation.
Isolation and Separation of DNA and RNA from a Single Tissue or Cell Culture Sample {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	We are currently developing a product using proprietary bead based technology that selectively binds DNA to the beads to effectively separate DNA and RNA independently. This allows for the extraction of all nucleic acids from one experimental setup
Isolation of DNA from Buccal Swabs using DNAdvance Kit	This protocol increases the volume of lysis buffer so that these two swabs from a single individual can be run in a single well and have the swab heads covered by lysis buffer.
High Recovery of DNA for Downstream Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt Genfind v2 system, genomic DNA isolation kit.
A workflow for medium-throughput isolation of cfDNA from plasma samples using Apostle MiniMax™ on the KingFisher™ Technology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of Apostle MiniMax™ High Efficiency cfDNA Isolation Kit, in conjunction with the KingFisher Duo Prime automated protein purification system.
High Recovery of DNA for Downstream Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt Genfind v2 DNA purification system isolates and purifies genomic DNA (gDNA) from whole blood and serum
Agencourt- CosMCPrep- and Agencourt CleanSEQ {D62418F5-2B8B-4155-AC0E-3ABFBF3E7911}	Génome Québec Innovation Centre discovers lower costs and improved processes.
DNAdvance DataSheet {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	The DNAdvance kit offers greater recovery of high quality gDNA than other commercially available DNA extraction kits. The extraction can be run manually in a 2 mL tube format or 96-well format, or automated in 96-well format on variety of Beckman Coulter Biomek liquid handling workstations.
GenFind V3 DataSheet {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	The method can be run manually in a 2 mL tube format or 96-well format, or automated in 96-well format on variety of Beckman Coulter Biomek liquid handling workstations
Manual or Automated RNA, DNA or Total Nucleic Acid Extraction from FFPE Tissues {279C6CA2-9797-4CFE-9CAA-5EAFAC29B60D}	FormaPure XL reagent kits represent a single-chemistry system with demonstrated capability for use in Next-Generation Sequencing (NGS) as well as other downstream applications including qPCR. Maximizing integrity, yield and purity from an FFPE sample is required to minimize the risk of losing important genetic information
Isolation of cell-free DNA (cfDNA) from plasma using Apostle MiniMax™ High Efficiency cfDNA Isolation kit comparison of fully automated, semi-automated and manual workflow processing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note compares workflows and yield for the extraction of cfDNA using Apostle MiniMax™ High Efficiency cfDNA Isolation Kit, following the manual protocol, automating the extraction using the Biomek i7 Hybrid Workstation, and semi-automating the extraction using the KingFisher™ Duo Prime Sample Purification System.
A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of FormaPure XL Total, a total nucleic acid extraction kit for FFPE samples, in conjunction with the KingFisher™ Duo Prime Sample Purification System as a potential solution that mitigates some of the challenges with FFPE workflows.
CosMCPREP DataSheet - High and Low Copy Plasmid Purification {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	CosMCPrep uses a single protocol to purify a variety of high and low copy number templates while maintaining the flexibility to support bothmanual and automated processing.
Apostle MiniMax Datasheet {7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}	Apostle MiniMax™ High Efficiency cfDNA Isolation Kit, Apostle MiniMax is a cell–free DNA (cfDNA) isolation reagent kit, built on magnetic bead–based technology. Apostle MiniMax has been demonstrated to purify cfDNA from human plasma in both manual and automated workflows.

Выделение ДНК из FFPE ткани

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FormaPure XL DNA Visual Workflow

FormaPure XL DNA Features

Product Specifications: FormaPure XL DNA

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