Myeloid Activation Assay Staining Protocol
This protocol is for reference purposes only. It may be necessary to adapt the method for specific research needs.
IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody Cocktail, Part Number C63854
MATERIALS REQUIRED, BUT NOT PROVIDED
Whole blood collection tubes with EDTA or Heparin anticoagulant
12 x 75 mm test tubes (Part Number 2523749)
Pipettors and pipet tips capable of delivering 10 and 500 µL
VersaLyse Solution (Part Number IM3648)
IOTest 3 Fixative Solution, 10X concentrate (Part Number IM3515)
Dilute IOTest 3 Fixative Solution 1:220 in VersaLyse Solution
Activators used for positive control
- Add 5 to 25 μL of whole blood to test tube.
- Add 10 μL of the IOTest CD169-PE/ HLA-DR-APC/ CD64-PB cocktail.
- Add 500 μL of VersaLyse/Fixative.
- Incubate 10 minutes.
- Acquire data with flow cytometer.
Note: For heparin anti-coagulated blood, stained samples can be acquired for up to 6 hours. For EDTA anti-coagulated blood, stained samples can be acquired for up to 3 hours.
See Example Data