Проточный цитометр CytoFLEX

до 5 каналов синего лазера (488 нм), до 3 каналов красного лазера (638 нм) и до 5 каналов фиолетового лазера (405 нм)
широкий спектр конфигураций: от 1 лазера и 4 флуоресцентных параметров до 3 лазеров и 13 флуоресцентных параметров
13 переставляемых полосовых оптических фильтров
фиксированная встроенная оптика и кварцевая проточная ячейка
непревзойденная чувствительность лавинных фотодиодов
абсолютная линейность детекторов обуславливает новые подходы к компенсации
канал бокового рассеяния фиолетового лазера (405 нм) для исследования микрочастиц
легкая активация дополнительных детекторов заменой USB-ключа

Ключевые характеристики

Подача образца

Полуавтоматический загрузчик пробирок 12 x 75 мм (5 мл) или микропробирок объемом 1,5 мл и 2,0 мл
Опциональный загрузчик 96-луночных планшетов
Волюметрическое измерение концентрации
Гидравлическая система обеспечивает постоянную скорость подачи образца

Компенсация

Инновационная библиотека компенсаций - хранилище коэффициентов перекрывания флуоресценции красителей для определения правильной компенсационной матрицы для любых настроек усиления и комбинаций красителей
Абсолютная линейность коэффициентов усиления позволяет использовать одни и те же настройки компенсации в разных экспериментах и для разных образцов

ПО CytExpert

Визуализация планшета в виде тепловых карт
API (интерфейс прикладного программирования) позволяет внешнему ПО управлять функциями загрузчика планшетов и запускать анализ для интеграции CytoFLEX в автоматизированные станции
Три варианта установки в зависимости от требуемого уровня защиты данных

Чувствительность и разрешение

FITC: < 30 MESF (молекул эквивалентного растворимого флуорохрома)
PE: < 10 MESF
Разрешение частиц < 200 нм с помощью канала фиолетового бокового светорассеяния (VSSC)
Исключительная чувствительность лавинных фотодиодных детекторов (APD) по отношению к световому излучению всего видимого диапазона

Технические характеристики

Detection	Photodiodes
Resolution	<300 nm particles (488 nm side scatter), <80 nm polystyrene particles (405 nm side scatter), rCV 3%
Performance	<30 MESF for FITC, <10 MESF for PE
Flow Cell	Patent-pending, alignment-free integrated optics quartz flow cell (420 μm x 180 μm ID) design with >1.3 numerical aperture
Signal Processing	Digital system with 7-decade display

Power Requirements	100-240 VAC, 50/60 Hz
Item Specifications Referenced	B53000

Документация

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Title	Description
How to use VSSC on CytoFLEX Technical Note {749D68FB-4893-4B2D-BD6C-C662F0520F64}	The purpose of this paper is to demonstrate how to setup the CytoFLEX flow cytometer to detect small particles by Violet Side Scatter (VSSC). A bead mixture is defined to assist in demonstrating the detection capabilities. Using VSSC, the CytoFLEX was able to clearly discriminate 80 nm polystyrene and 150 nm silica nanoparticles from background noise.
Red Blood Cell Microparticle Analysis by Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Discover how to stain microparticles for flow cytometric analysis. The assay describes sample preparation, staining, and gating steps to distinguish red blood cell-derived microparticles from microparticles from other tissue origins. Representative data from several replicates are provided in order to report out precision obtainable from this approach.
Single Cell Analysis of Autophagy Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Provides a detailed procedure for using the Cyto-ID Autophagy kit in a flow cytometry assay. Contains background information on the physical and chemical hallmarks of autophagy and the benefits of a single cell assay versus bulk analysis of this biological process.
Competitive Mouse Bone Marrow Transplantation Reconstitution Assay Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Using primary-conjugated antibodies against CD45.1 and CD45.2, we measure competitive bone marrow transplantation success by quantifying the proportion of donor cells (marked by CD45.1/Ly5.1 expression) and recipient cells (marked by CD45.2/Ly5.2 expression) in the peripheral blood of irradiated recipient mice post-transplantation.
Calcium Flux Assay by Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The unique peristaltic sample delivery system of the CytoFLEX analyzer enables optimized measurements of transient changes in intracellular calcium in cells following agonist activation.
Flow Cytometric Analysis of Auto-fluorescent Cells in Marine Demosponge Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Natural autofluorescence of marine organisms can be detected in flow cytometers. Utilizing this, an assay to assess aggregation and reorganization of the sponge is described.
Using Flow Cytometry to Assess Protein Aggregation in Bacterial Overexpression Systems Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes the use of Proteostat dye in a flow cytometric assay for detection of aggregated protein in bacteria inclusion bodies. This assay can be used to assess bacterial culture conditions that favor production of soluble protein. This method reduces the amount of sample preparation required compared to gel electrophoresis-based methods.
Measuring Proliferation in Leukemia Cell Lines Using CFSE Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye which is cell permeable. It binds covalently to intracellular proteins. Fluorescence decreases over time as the dye is divided between daughter cells at each division. In this application note a detailed protocol for using this assay to assess cytotoxic effects of a pharmacologic agent is described.
Thirteen Color Immunophenotyping T Cell Subsets in Whole Blood Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Contains a comprehensive overview of T cell biology and the implications for multicolor immunophenotyping panel design. Includes a detailed gating strategy for deep immunophenotyping of T cell populations including rare cell types. Also includes step-by-step instructions for setting up the CytoFLEX for multicolor immunophenotyping including use of the Compensation Library.
Eight Color Immunophenotyping Unlysed Whole Blood Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This whole blood assay does not involve harsh lysis or centrifugation steps, minimizing any cell perturbations from sample handling. This 8-color panel provides a broad survey of peripheral blood leukocytes.
Identification of Natural Killer Cells in Rat Placenta Model of Preeclampsia Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	A special population of natural killer cells (uNK) has been implicated in preeclampsia. uNK cells have contact with the trophoblast, modify uterine arteries to promote blood flow, and modulate trophoblast invasion in a dose-dependent manner. In this application note a detailed method for preparing resident NK cells from tissue and an assay to access NK cell activation is provided.
Phagocytosis of Bacteria in a Whole Blood Assay Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes an opsonization assay which can be used to assess the phagocytic capabilities of immune cell in whole blood. The benefits of using multicolor flow cytometry for the assay is that different phagocytic potential across various immune cell types can be determined in the same sample.
Using Cell Impermeant DNA Binding Dyes for Viability Assessment in Multicolor Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Compares viability staining using DRAQ7, DAPI, and 7-AAD in a multicolor immunophenotyping panel. Attributes of each dye are evaluated to aid in selection during panel design.
CytoFLEX Instrument Set-up for Measuring Extracellular Vesicles Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes the instrument set up and verification steps required for measuring extracellular vesicles by flow cytometry. Instructions for reducing background particles found in buffers and reagents to enhance small particle detection.
BeckmanConnect Security Highlights {749D68FB-4893-4B2D-BD6C-C662F0520F64}	This document highlights the privacy and security features in the BeckmanConnect remote management solution.
Beckman Coulter Instrument PC Networking Requirements {749D68FB-4893-4B2D-BD6C-C662F0520F64}	This document describes the minimum technical criteria required to connect a Beckman instrument PC to a customer’s local network and/or the internet.
Absolute Counting using the CytoFLEX {E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}	In this whitepaper we compare and contrast syringe-based and peristaltic pump-based fluidics system for the application of beadless absolute counting. Topics important to the validation of a counting assay are discussed.
Tech Note: Violet Side Scatter Use in Nanoparticle Detection {749D68FB-4893-4B2D-BD6C-C662F0520F64}	How to Use Violet Side Scatter to Detect Nanoparticles on the CytoFLEX Flow Cytometer
Assessing T Cell Subpopulations by TCR Expression in PBMCs Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We outline a protocol using DURAClone™ IM panels, which are unitized, dry format, room temperature stable reagent cocktails, to delineate and quantify T cell subpopulations from a heterogeneous sample of isolated normal PBMCs based on TCR composition.
Ten Color Immunophenotyping of Mouse NK Cells Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Surface-marker expression is critical to the delineation of NK cell maturity, function, and localization. We present a protocol and gating strategy for the identification of splenic murine NK cell populations via the CytoFLEX flow cytometer using a 10-color screen.
Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this case study, using the surface markers Ly6C (hematopoietic precursor cells, monocytes), Ly6G (granulocytes/neutrophils), and CD115 (macrophage colony-stimulating factor [M-CSF] receptor; macrophages), we examined and identified the differences in circulating cell populations in fresh blood samples.
Flow Cytometric Quantification to Assess Doral Endocytosis Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of flow cytometry to examine the phagocytosis of collagen type I-coated fluorescent microspheres by rat embryonic fibroblasts. Cellular uptake can be assessed quickly in a large number of cells using flow cytometry.
Characterizing T Helper Cells Populations by Intracellular Cytokine Expression Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	T helper cells are distinguished functionally by their cytokine profile, their major effector molecules. This application note provides a procedure for assessing the cytokine profile using flow cytometry and demonstrates T Helper cell subtypes Th1, Th2, Th17 and a non-classic Th1 type.
Identifying Discrete Mouse Splenocyte and Peripheral Blood Cell Subpopulations Using Surface Markers Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Flow cytometry is extremely useful for detecting cell surface antigen expression, and thereby facilitates the characterization, identification, and isolation of discrete cell populations within heterogeneous samples. We describe a method to rapidly isolate and delineate CD3ε-positive T cell and CD19-positive B cell populations from murine spleen and peripheral blood samples.
Phenotypic Characterization of Human Natural Killer Cell Populations in Peripheral Blood Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes a 10 color, 13 marker panel to investigate the NK cell population, both phenotypically and functionally in human PBMC samples. The panel includes the use of an exclusion markers on a dump channel used to gate out non-NK cells. Deep phenotyping markers for the NK cell compartment are included and a comprehensive gating strategy is defined.
Identifying Brain Tumor Initiating Cells Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Tumors represent a heterogenous population. Enumerating cells with stem cell like properties via co-expression of markers provides insight into the evolution of the tumor over time and throughout treatment. Detailed procedure for processing brain tissue for culture and flow cytometric analysis is provided.
Plant Genome Analysis by Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Provides a detailed protocol for preparing nuclei from plant tissues. DNA in the nuclei is then stained with propidium iodide and the flow cytometer is used to assess DNA content. Values obtained are then correlated with the Kew Database to assess ploidy. This assay can be used to investigate the role of endoreplication in plant development across species.
Flow Cytometric Approach to Probiotic Cell Counting and Analysis Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes a staining procedure and instrument set up for absolute counting of probiotic bacterial culture. A workflow comparison with the traditional plate-based assay as well as correlated cell counts are provided.
Specification Sheet: CytoFLEX Series V-B-R {B14306BD-BB46-4B27-BF3E-B11862085BC7}	Technical Specifications for the CytoFLEX Violet-Blue-Red (V-B-R) Series of flow cytometers
CytoFLEX Flow Cytometer Platform Join the Resolution Revolution {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	The CytoFLEX Platform is a revolutionary system presenting optimal excitation and emission, minimizing light loss and maximizing sensitivity
Protection Plan for your Workflows in Flow Cytometry {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Maintenance service plan for Beckman flow analyzers.
Flow Cytometry Training {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Real-world training programs to maximize the value of your flow cytometer investment.
BeckmanConnect Installation and Activation Instructions {B14306BD-BB46-4B27-BF3E-B11862085BC7}	A step-by-step guide to help you successfully install BeckmanConnect.
Quality Assurance for your CytoFLEX Flow Cytometer {A0D0EF0B-43F2-431C-BEB0-69F98765BDC4}	Paramount for any lab is consistent accurate data. Proper instrument performance is central to achieving this.
Manufacturing Processes for Engineered T-Cell Therapy – CAR-T	Brochure presenting the CAR-T cell therapy workflow and associated product portfolio
Twenty-seven Markers on a 3-laser Flow Cytometer {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	This poster was presented at Cyto in 2018 and demonstrates a technique to maximize the usage of channels on a flow cytometer. The method relies on stacking biomarkers that are not expressed on the same cell and a gating technique to interrogate the populations of interest.
Nano- and Microparticle Bead Mix for Use as a Sizing Standard in Flow Cytometry {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	The CytoFLEX platform is distinguished for not only its fluorescence sensitivity but also its ability to detect in the nanoparticle range using Violet Side Scatter. This poster, presented at Cyto in 2016 describes a NIST-traceable bead mix for use in standardizing flow cytometry experiments.
Resolving Dyes with Identical Emission Wavelengths on the CytoFLEX {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Compensation in flow cytometry is a special case of spectral deconvolution. In this poster we demonstrate that the CytoFLEX can deconvolute highly overlapping fluorophores using the compensation algorithm.
Using Violet Side Scatter to Detect and Resolve Nanoparticles on the CytoFLEX Flow Cytometer {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	This poster provides detailed instructions for setting up the CytoFLEX flow cytometer Violet Side Scatter for nanoparticle detection. Experimental considerations for nanoscale flow cytometry and illustrated with data from the instrument.
CytoFLEX Instrument Evaluation Using Biological Specimens {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: James Tung, Dan Condello, Albert Donnenberg, Erika Duggan, Jesus Lemus, John Nolan, Kathy Ragheb, Jennifer Sturgis, Paul Scibelli, J Paul Robinson, Domenic Fenoglio \| Beckman Coulter, Miami FL, Purdue University Cytometry Laboratories, La Jolla Bioengineering Institute, University of Pittsburgh Cancer Center